We narrowed to 2,664 results for: calcium

1. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLYS5-MCU-Flag

   Plasmid

   #50054

   Purpose

   Expression of human MCU in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   MCU-Flag (MCU Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human NCKX3-FlagTag (pcDNA3.1-)

   Plasmid

   #75206

   Purpose

   Mammalian expression of SLC24A3

   Depositor

   Jonathan Lytton

   Article

   Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

   Insert

   SLC24A3 (SLC24A3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-Soma-jGCaMP8f

   Plasmid

   #169258

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   Soma-jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT2 Beta1b GFP

   Plasmid

   #89893

   Purpose

   Mammalian expression of Beta1b GFP

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   GFP sequence inserted just before the Stop codon

   Promoter

   adenovirus major late promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT2 Beta1b mCherry

   Plasmid

   #89892

   Purpose

   Mammalian expression of Beta1b mCherry

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mCherry sequence inserted just before the Stop co…

   Promoter

   adenovirus major late promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLYS1-EMRE/RNAi resistant

   Plasmid

   #50056

   Purpose

   Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   EMRE-RNAi resistant (SMDT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNAi resistant, silent mutant

   Promoter

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuex2-iBB-GECO1

   Plasmid

   #194216

   Purpose

   Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

   Depositor

   Robert Campbell

   Article

   Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

   Insert

   iBB-GECO1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSynapsin1-axon-GCaMP6f

   Plasmid

   #112000

   Purpose

   For enriched expression of GCaMP6f in axons

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV1

   Insert

   axon-GCaMP6f
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-4mtD3cpv

    Plasmid

    #181874

    Purpose

    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    4mtD3cpv
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    the 4mtD3cpv CDS differs from the recorded GeneBa…

    Promoter

    CaMKIIa

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MUNC18-2

    Plasmid

    #186687

    Purpose

    Expresses MUNC18-2 in insect cells

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    MUNC18-2 (Stxbp2 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

    Plasmid

    #163881

    Purpose

    Acute Depletion of Mau2-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Mau2 (Mau2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-MFRN2

    Plasmid

    #50546

    Purpose

    Expression of MFRN2 gene in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    Mrfn2 (SLC25A28 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

16. GFP-Myo 1d

    Plasmid

    #134906

    Purpose

    Expression of rat Myo 1d (Myr 4) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

    Insert

    Myo 1d (rat) with 3' UTR (Myo1d Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNAP-23

    Plasmid

    #186686

    Purpose

    Expresses SNAP-23 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    SNAP23
    

    Use

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSynapsin1-axon-GCaMP6m

    Plasmid

    #111261

    Purpose

    For enriched expression of GCaMP6m in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6m
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-CAD

    Plasmid

    #73567

    Purpose

    Soluble activator of Orai1 channels

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    CAD (STIM1 342-448) (STIM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330 NIPBL gRNA

    Plasmid

    #165590

    Purpose

    Insertion of NIPBL degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    NIPBL (Nipbl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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