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Showing: 1381 - 1400 of 12857 results

  2. dTomato_LD 0_CS M_tTA-BFP

    Plasmid
    #58875
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58875-plasmid-map-sequence-id-94236

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  5. NmMetQ N238A

    Plasmid
    #129648
    Purpose
    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence
    Depositor
    Douglas Rees
    Article
    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p
    Insert
    metQ Gene
    Use
    Tags
    10x Histidine Tag, Enterokinase, and pelB signal …
    Expression
    Bacterial
    Mutation
    Changed Asparagine 238 to Alanine
    Promoter
    T7 Promoter
    Available since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    129648-plasmid-map-sequence-id-254340

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  13. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

    Plasmid
    #162918
    Purpose
    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162918-plasmid-map-sequence-id-330526

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  15. jk64

    Plasmid
    #87012
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    Nrx1B-4xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87012-plasmid-map-sequence-id-173035

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  16. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

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Showing: 1381 - 1400 of 12857 results