We narrowed to 7,257 results for: trac

1. AAV-hGFAP-mCherry-shLuci

   Plasmid

   #178588

   Purpose

   Expression of mCherry and a shRNA against luciferase under the human GFAP promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   miRE-Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM310-Myc

   Plasmid

   #80053

   Purpose

   Expression of a Myc tagged version of the BlaM-Vpr fusion protein

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   Myc-BlaM-Vpr
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   N-terminal Myc tag added to construct pMM310

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSIIbsr-GCaMP6s

   Plasmid

   #184712

   Purpose

   Calcium biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mScarlet-TEV-Strep-His

   Plasmid

   #210823

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mScarlet
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIS.MTRIA-AVP

   Plasmid

   #184600

   Purpose

   For expression of a fluorescent sensor for vasopressin in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-AVP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HiBiT-NbV5

   Plasmid

   #201490

   Purpose

   NanoBit detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   HiBiT-NbV5
   

   Use

   Tags

   HiBit

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24_NusA-Tus

   Plasmid

   #165959

   Purpose

   Expression of soluble E. coli Tus protein

   Depositor

   Keith Pardee

   Article

   Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

   Insert

   Tus
   

   Use

   Tags

   His-NusA-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF-N-HiBiT-template

   Plasmid

   #200882

   Purpose

   template for N-terminal HiBIT-tagging

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HiBIT-linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. cSPBN-4GFP

    Plasmid

    #52487

    Purpose

    Expresses EGFP

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Protoc. 2010 Mar;5(3):595-606. doi:

    Insert

    EGFP
    

    Use

    Rabies virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

    Plasmid

    #137994

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-GCaMP6fu

    Plasmid

    #86235

    Purpose

    Express fluorescent calcium indicator GCaMP6fu in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

    Insert

    GCaMP6fu
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W43Y D395A

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. prCAG-BoxB

    Plasmid

    #191122

    Purpose

    Transcription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoter

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Inserts

    rCAG
    BoxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tetracycline-inducible promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-KIRIN1-GR

    Plasmid

    #107417

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1-GR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-cPLA2-mEGFP

    Plasmid

    #184718

    Purpose

    Exogenous cPLA2WT

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    cPLA2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSIIpuro-4493NES(Booster-PKA)

    Plasmid

    #184710

    Purpose

    PKA biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    Booster-PKA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIEx4-Dio-GFP

    Plasmid

    #198278

    Purpose

    Cre reporter for insect cells; double-floxxed-EGFP

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pIEx4-Dio-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIS.MTRIA-PGE

    Plasmid

    #184617

    Purpose

    For expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-PGE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid

    #212122

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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