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pENTR R4-QF-R3

Plasmid

#32310

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

QF

Use

Gateway multisite entry clone

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3FLAG

Plasmid

#221045

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xFLAG epitopes

Use

Unspecified

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pME-QF2w-SV40pA

Plasmid

#155117

Purpose

Middle entry vector containing QF2w

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QF2w

Use

Gateway middle entry vector

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT31

Plasmid

#89666

Purpose

expression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT31

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT27

Plasmid

#89665

Purpose

expression of doubleTAL staple protein dT27 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT27

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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SMX DEST vector

Plasmid

#62399

Purpose

Empty Backbone

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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BLA/V5

Plasmid

#1806

Depositor

Christian Tschudi

Publication

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

BLA/V5

Tags

V5

Expression

Bacterial

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only
pPOTv6-puro-3Ty:tagRFPt:3Ty

Plasmid

#201089

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Myc:mScarletI:3Myc

Plasmid

#179804

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Publication

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJK210

Plasmid

#73325

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Publication

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 (strong) promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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c7_HLL

Plasmid

#63125

Purpose

cysteine free Mm-cpn mutant

Depositor

Tanja Kortemme

Publication

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQCas12j2

Plasmid

#189780

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

Cas12j2

Use

CRISPR; Gateway compatible cas12j2 entry clone

Tags

NLS

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265

Plasmid

#164712

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Publication

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

Expression

Plant

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSB1K3_pBen-sfGFP_J23101-mRFP

Plasmid

#128126

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Publication

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen (benzoate responsive promoter)

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet1_CoiC_CoiSA

Plasmid

#208762

Purpose

Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Publication

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiC
CoiSA

Expression

Bacterial

Mutation

single nucleotide insertion (C70) at RBS of CoiC

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - APX

Plasmid

#89618

Purpose

Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Ascorbate dependent Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Phelo

Plasmid

#48995

Purpose

Gateway adapted transfer of Phleomycin resistance gene

Depositor

Rick Tarleton

Publication

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

Sh ble

Use

Gateway attb1 & attb2

Available since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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