We narrowed to 11,919 results for: try

1. mutpRL-TK 5xUTR

   Plasmid

   #40760

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   5 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

2. mutpRL-TK 1xUTR

   Plasmid

   #40756

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

3. CS2-Myc-dLIS1

   Plasmid

   #47047

   Purpose

   Expression of tagged Drosophila LIS1

   Depositor

   Laura Lee

   Article

   Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

   Insert

   Lis1 (Lis-1 Fly)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. CS2-CHY-dLIS1

   Plasmid

   #47045

   Purpose

   Expression of fluorescent Drosophila LIS1

   Depositor

   Laura Lee

   Article

   Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

   Insert

   Lis1 (Lis-1 Fly)
   

   Use

   Tags

   CHY

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pDM10

   Plasmid

   #41759

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S A378C C451S

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM8

   Plasmid

   #41758

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S S364C C451S

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOU1

   Plasmid

   #41753

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C338S C451S

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUB39

   Plasmid

   #26300

   Depositor

   George Cross

   Insert

   VSG117 (Lister 427-4) cDNA
   

   Use

   Trypanosome inducible expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Use

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-PGK-hESRRB

    Plasmid

    #60434

    Purpose

    Over-expression of human ESRRB

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    ESRRB (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-neoBeta2mcherry

    Plasmid

    #45097

    Depositor

    Henry Lester

    Article

    Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

    Insert

    nAChR beta2 mcherry (Chrnb2 Mouse)
    

    Use

    Tags

    has c-myc tag

    Expression

    Mammalian

    Mutation

    β2 was cloned into pCI-neo at the restriction sit…

    Promoter

    CMV

    Available since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-betaPIXa

    Plasmid

    #15234

    Depositor

    Rick Horwitz

    Article

    Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

    Insert

    beta-PIXa (Arhgef7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-ECFP-betaPIXa

    Plasmid

    #15235

    Depositor

    Rick Horwitz

    Article

    Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

    Insert

    beta-PIXa (Arhgef7 Mouse)
    

    Use

    Tags

    ECFP and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA-mSin1(1.1)-HA

    Plasmid

    #73388

    Purpose

    Expresses mSin-HA

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

17. EGFR-ET1

    Plasmid

    #44185

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    EGFR-ET1 (EGFR Human)
    

    Use

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    I104V and D254N relative to NP_005219.2

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR221-FGFR4

    Plasmid

    #60531

    Purpose

    Entry vector for human FGFR4

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    c2134a P712T

    Promoter

    NA

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pPB-hCMV1-hKLF2-IV

    Plasmid

    #60433

    Purpose

    tet-inducible human KLF4

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-PGK-hKLF4

    Plasmid

    #60435

    Purpose

    Over-expression of human KLF4

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge