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pCMU-ACTFr

Plasmid

#61191

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P05

Plasmid

#120016

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

CUP1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pWM_12x601_30bpLinker

Plasmid

#157789

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_30bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Tags

3xHA

Expression

Bacterial and Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSF278

Plasmid

#167673

Purpose

Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Mut2-RS

Plasmid

#182882

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut2-RS

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PmxaF

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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