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Addgene
Showing: 2131 - 2160 of 5330 results
  1. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Insert
    CACNA1D (Cacna1d Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pDragon-Ctgf

    Plasmid
    #155015
    Purpose
    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.
    Insert
    Ccn2 (Ccn2 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    M24T (please see depositor comments)
    Promoter
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pRD405

    Plasmid
    #163110
    Purpose
    Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Insert
    NLS_mScarlet-I_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. Pyr pUAST-HA

    Plasmid
    #69768
    Purpose
    Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Insert
    Pyramus (FGF8like-2) (pyr Fly)
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    241T amino acid insertion compared to reference s…
    Promoter
    hsp70 promoter
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pTXB1-hMeCP2-R270X

    Plasmid
    #48092
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270X
    Depositor
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pTXB1-hMeCP2-G273X

    Plasmid
    #48093
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X
    Depositor
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2131 - 2160 of 5330 results