We narrowed to 6,657 results for: sas

1. P14-pZE-SUMO-ObiH

   Plasmid

   #204629

   Purpose

   ColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

   Depositor

   Aditya Kunjapur

   Article

   Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

   Insert

   His-SUMO-TEV-ObiH
   

   Use

   Synthetic Biology

   Tags

   His Tag; SUMO Tag; TEV Protease Site

   Expression

   Bacterial

   Promoter

   PLtetO-1 promoter

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV mSin1.2 HA

   Plasmid

   #12583

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mSin1.2 (MAPKAP1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pMSCV mSin1.1 myc

   Plasmid

   #12576

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mSin1.1 (MAPKAP1 Human)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

4. p4A8_S7T_BC

   Plasmid

   #217832

   Purpose

   4A8 variant in barcoded, yeast surface display plasmid

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   4A8 variant Fab
   

   Expression

   Yeast

   Mutation

   VL: S7T

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_CaMKIIa_SERT_EYFP

   Plasmid

   #198737

   Purpose

   Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

   Depositor

   Lin Tian

   Article

   Vargas et al Science. 2023 Feb 17;379(6633):700-706.

   Insert

   Serotonin transporter and yellow fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SauCas9 PE2*

   Plasmid

   #169851

   Purpose

   Mammalian Expression, sauCas9 based prime editor

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SaCas9-N580A-M-MLV
   

   Tags

   bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-sh-mSOX9-2

   Plasmid

   #40645

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSOX9-2 (Sox9 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNAintron-CHIKV-nsp3-c3xFLAG

   Plasmid

   #215696

   Purpose

   Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp3
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pWaldo-GFPe_PepTSo

   Plasmid

   #58334

   Purpose

   Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

   Depositor

   Simon Newstead, So Iwata

   Article

   Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

   Insert

   PepTso
   

   Tags

   GFP-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF-Ivy-his-hLYZ

    Plasmid

    #83920

    Purpose

    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Ivy
    lysozyme (LYZ Human)
    

    Tags

    Ivy protein has C-terminal hisx6 tag

    Expression

    Bacterial

    Mutation

    Ivy leader sequence deleted and replaced.

    Promoter

    T7 and t7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-sh-mSlug-4

    Plasmid

    #40648

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-4 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. SB-SRT-Puro

    Plasmid

    #154888

    Purpose

    Sleeping Beauty self-reporting transposon with puromycin reporter

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    Sleeping beauty SRT
    

    Use

    Transposon

    Expression

    Mammalian

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GST FNIP2

    Plasmid

    #164982

    Purpose

    FNIP2 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FNIP2 (FNIP2 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. B52 + SMARCAL1 sgSTOP

    Plasmid

    #100715

    Purpose

    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Venus-MDGA1

    Plasmid

    #82543

    Purpose

    Overexpression vector for Venus-MDGA1 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA1 (MDGA1 Human)
    

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Promoter

    chicken beta actin

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV_pSyn_Mito-LOV-Turbo-V5

    Plasmid

    #199669

    Purpose

    expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    COX4I1 mito targeting sequence and V5

    Promoter

    Synapsin

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 FLAG DEPTOR (PDZ domain)

    Plasmid

    #21701

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR PDZ domain (DEPTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PDZ domain only

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

    Plasmid

    #107327

    Purpose

    Expresses SpCas9 fused to dNmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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