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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 92 results for: sas

Showing: 1 - 20 of 92 results

  1. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs... c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some...use a type V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type...

  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...of sample types (Engvall 1971). Scientists value ELISAs for their accuracy, specificity, and sensitivity...considerations when using the method. ELISA basics All ELISAs follow the same overall process. First users coat...figure was created with BioRender.com  Direct ELISAs A direct ELISA is the quickest and simplest of ...saves time, it also has several drawbacks. Direct ELISAs generally have higher levels of background staining...components and increase background staining. Direct ELISAs also lack the signal amplification afforded by ...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of...also be a source of cross-reactivity.  Sandwich ELISAs In the most common type of ELISA, the sandwich ...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid...transcript. By Arunreginald at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-pairing.../doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical analysis of codon optimization ...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria meningitidis (Nm ...K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically...XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling...Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier...

  14. Science Career Options

    Type
    Blog Post
    ...PowerPoint etc) or Programming skills (Python, R, SAS, HTML, Matlab etc) are highly desirable in a range...

  15. Components of CRISPR/Cas9

    Type
    Blog Post
    ...or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus...

  16. Summer SciComm: Preprints

    Type
    Blog Post
    ...Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license. Many scientists and advocates have argued...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

  20. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable and reversible...
Showing: 1 - 20 of 92 results