...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs... c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Type...use a type V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type...

...Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane...

...27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling...Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier...

...Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP. Nat Methods...

...GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp...Tn7-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions...recruits the transposase proteins to the target location in the genome, where the transposase then carries...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases..., and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems like piggyBac or Tn7 allow... elements into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) ...) combine the large-scale capacity of transposases with the precise DNA-targeting of CRISPR-Cas systems...

...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...verified to contain your protein of interest (in ELISAs, the standard curve can serve as the positive control...

...ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker ALS Sascha Martens...mCherry ALS Sascha Martens 190192 pET-Duet1-6xHis-TEV-OPTN OPTN His, TEV, mCherry ALS Sascha Martens 190486...ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha Martens...GST, Tev P10 ALS Sascha Martens 198428 pGST2-GST-TEV-OPTN OPTN TEV, GST tac ALS Sascha Martens 199205 pBMN-HA-TBK1... TBK1 HA ALS Sascha Martens 199206 pBMN_HA-TBK1 (kinase dead - D135N) TBK1 HA ALS Sascha Martens 199207...GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens 209078 ...199207 pBMN_HA-TBK1 (E696K) TBK1 HA ALS Sascha Martens 199399 Anti-PINK1 [N4/49R] PINK1 CMV Parkinson's James...

... 137864 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) iCre-ERT2 (PAGSAS N-terminus) - Tamoxifen inducible CAG ...

...generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional...

...Use a CRISPRi system to target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural oxytocin...

...TGCCAGCACAATAGATTGcaatggcaccgggttgGCAAGAGATCGCGTCGCA musashi-2a TAL3122 & TAL3123 TGGAAGCGGACGCGAGTCaagtcacctctggaagCCTGAACGATTCGCAACA...

...glucose phosphate isomerase AMF, GNPI, NLK, PGI, PHI, SA-36 GPR17 G protein-coupled receptor 17 DKFZp686M18273...