We narrowed to 7 results for: sas

...Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases (CASTs) combine...tables below for CRISPR transposase plasmids. To learn more about CRISPR transposases and other CRISPR topics...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...combine the large-scale capacity of transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs... c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some...

...27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling...Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier...

...GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp...Tn7-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions...recruits the transposase proteins to the target location in the genome, where the transposase then carries...Figure 7: Overview of CRISPR transposases. Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases..., and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems like piggyBac or Tn7 allow... elements into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) ...) combine the large-scale capacity of transposases with the precise DNA-targeting of CRISPR-Cas systems...

...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...verified to contain your protein of interest (in ELISAs, the standard curve can serve as the positive control...

...ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker ALS Sascha Martens...mCherry ALS Sascha Martens 190192 pET-Duet1-6xHis-TEV-OPTN OPTN His, TEV, mCherry ALS Sascha Martens 190486...ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha Martens...GST, Tev P10 ALS Sascha Martens 198428 pGST2-GST-TEV-OPTN OPTN TEV, GST tac ALS Sascha Martens 199205 pBMN-HA-TBK1... TBK1 HA ALS Sascha Martens 199206 pBMN_HA-TBK1 (kinase dead - D135N) TBK1 HA ALS Sascha Martens 199207...GFP CMV ALS Sascha Martens 208867 pHAGE-FKBP-GFP-OPTN (2-119) OPTN GFP, FKBP CMV ALS Sascha Martens 208875...199207 pBMN_HA-TBK1 (E696K) TBK1 HA ALS Sascha Martens 199399 Anti-PINK1 [N4/49R] PINK1 CMV Parkinson's James...

...generates large genomic deletions. Insert CRISPR transposases insert large fragments of DNA. Transcriptional...

...glucose phosphate isomerase AMF, GNPI, NLK, PGI, PHI, SA-36 GPR17 G protein-coupled receptor 17 DKFZp686M18273...