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pDGB3_omega1R

Plasmid

#68240

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega2R

Plasmid

#68241

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119

Plasmid

#66136

Purpose

Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(T214V)

Plasmid

#66137

Purpose

Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 T180C

Plasmid

#66181

Purpose

Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C

Plasmid

#66180

Purpose

Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C

Plasmid

#66176

Purpose

Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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