Addgene

We narrowed to 2,257 results for: Beta-s

Showing: 1461 - 1480 of 2257 results

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pBiT3.1-N_MLST8:1-326

Plasmid

#211216

Purpose

Mammalian expression of full-length human MLST8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MLST8:M1-G326 (MLST8 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_TBL2:70-405

Plasmid

#210936

Purpose

Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TBL2:E70-H405 (TBL2 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-C_MLST8:1-326

Plasmid

#211290

Purpose

Mammalian expression of full-length human MLST8. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MLST8:M1-G326 (MLST8 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2-hCM1

Plasmid

#196863

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Akna-mEGFP

Plasmid

#196866

Purpose

Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mEGFP (Akna Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2-rCM1

Plasmid

#196861

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2-rCM1 (Nedd1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2-F75A

Plasmid

#196862

Purpose

Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2-F75A (Nedd1 Rat)

Use

Tags

Expression

Mammalian

Mutation

F75A in the CM1 domain

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Akna-mOrange2

Plasmid

#196865

Purpose

Expression of the centrosomal protein Akna fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mOrange2 (Akna Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVbsd-PPARD-H413W-K421A-K422A

Plasmid

#130765

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD H413W-K421A-K422A (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

H413W-K421A-K422A

Promoter

Available since

Aug. 27, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pPBpuro-mNG-eDHFR(69K6)-p85iSH2

Plasmid

#172103

Purpose

Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Article

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Use

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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NG1301 pCI-TPI-4MS2-SMG5(short)-4H

Plasmid

#65806

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4 MS2 stem loops, SMG5 3'UTR shortened versi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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FR_HNF1A

Plasmid

#31104

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

HNF1A (HNF1A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Use

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN

Plasmid

#116780

Purpose

Lentiviral expression of PTEN

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-NEUROD1

Plasmid

#215031

Purpose

Mammalian expression of human NEUROD1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

neuronal differentiation 1 (NEUROD1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-Cdk5rap2

Plasmid

#196859

Purpose

Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-R130Q

Plasmid

#116631

Purpose

Lentiviral expression of PTEN R130Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

Expression

Mutation

R130Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-Y336*

Plasmid

#116638

Purpose

Lentiviral expression of PTEN Y336*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

Expression

Mutation

Y336*

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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