We narrowed to 81,810 results for: Mycs
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Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRExpressionMammalianAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF3∆165N (H348E)
Plasmid#175420PurposeEncodes His-MBP-TEV-ybbR-H348E eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
TagsHis-MBP-TEV and ybbRExpressionBacterialMutation∆165N, H348EPromoterT7Available SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-NKD2S31A
Plasmid#174331Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsMycHisExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
TagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN071
Plasmid#166729PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN072
Plasmid#166730PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
Plasmid#164916PurposeExpresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTRDepositorInsertGCN4(UTR)-ZEM(TF)
UseSynthetic BiologyExpressionYeastPromoterPGK1 (yeast)Available SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPS1-Venus
Plasmid#133118PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.DepositorInsertpYPS1-YFP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only