We narrowed to 2,567 results for: gem

1. pCMV-lyso-pHoenix

   Plasmid

   #70112

   Purpose

   Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
   

   Tags

   Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Synapsin-pHoenix-WPRE

   Plasmid

   #70111

   Purpose

   lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   pHoenix (Syp Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
   

   Use

   Lentiviral

   Tags

   Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

   Promoter

   Synapsin

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB C-term-mScarlet-Blast

   Plasmid

   #169221

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Blast
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-DIO-somBiPOLES-mCerulean

   Plasmid

   #154951

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVBB C-term-mScarlet-Puro

   Plasmid

   #169220

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TVBB C-term-mNeongreen-Puro

   Plasmid

   #169228

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaMKII-somBiPOLES-mCerulean

   Plasmid

   #154948

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV-EF1aGFP2AKAT7-W

   Plasmid

   #159292

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 (KAT7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10)

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. hSyn-GtACR2-ChRmine-tandem-eYFP

   Plasmid

   #192580

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   EYFP- soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TVBB C-term-mNeongreen-Blast

    Plasmid

    #169229

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Blast
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

    Plasmid

    #110302

    Purpose

    Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2R3a-VenusYFP-3AT

    Plasmid

    #71269

    Purpose

    Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    4xGly linker and T3A pea Pisum sativum ribulose-1…

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid

    #113961

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-563 fused to a transmembrane tether

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

    Plasmid

    #118274

    Purpose

    Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Inserts

    bPAC(WT)
    SthK
    

    Use

    AAV

    Tags

    His tag, P2A, mCherry, and myc tag

    Expression

    Mammalian

    Promoter

    CamK II

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. TVBB C-term-mTurquoise2-Hygro

    Plasmid

    #169225

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Hygro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid

    #206259

    Purpose

    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    GTS mTagBFP
    

    Use

    Multimate/gateway entr 4

    Tags

    BFGT1 (Golgi localisation peptide)

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMMACE DEST CMV H2B iRFP

    Plasmid

    #206253

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713
    

    Use

    Recombinant baculovirus production, multimate/gat…

    Tags

    iRFP713

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMMK ENTR 2 CMV mKO2-hCdt1

    Plasmid

    #206282

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mKO2-hCdt1 (CDT1 Human)
    

    Use

    Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TVBB C-term-mTurquoise2-Puro

    Plasmid

    #169224

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV2_hSyn_Phobos_CA_Citrine

    Plasmid

    #98218

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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