We narrowed to 9,942 results for: plasmids 101

1. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

   Plasmid

   #132682

   Purpose

   AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Peptidase M20 domain containing 1 (Pm20d1 Mouse)
   

   Use

   AAV

   Tags

   6xHIS and Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169591

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin-2

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS1026-Tier1-PhCMV-3xNLS-iRFP670

   Plasmid

   #169532

   Purpose

   Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized far-red fluorescent protein iRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2381-Tier1-PhCMV-Igk_TM

   Plasmid

   #169487

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven membrane localization
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-OTp-hM3Dq-Myc

   Plasmid

   #184753

   Purpose

   To drive hM3D fused with Myc tag under the control of mouse oxytocin promoter

   Depositor

   Kazunari Miyamichi

   Article

   Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

   Insert

   hM3D (Gq)
   

   Use

   AAV

   Tags

   3xMyc

   Expression

   Mutation

   Promoter

   mouse Oxytocin promoter

   Available since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221-RAP1

   Plasmid

   #84525

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   none

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

   Plasmid

   #132684

   Purpose

   AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Peptidase M20 domain containing 2 (Pm20d2 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGDP3 aph(3'')-Ia

   Plasmid

   #112879

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-rap1-K651R

   Plasmid

   #84528

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K651R

   Promoter

   none

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TtgO2-PCMVmin-2

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

    Plasmid

    #169589

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid

    #169530

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

    Plasmid

    #169590

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS2392-Tier1-PhCMV-p2a_medFT

    Plasmid

    #169529

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169667

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    VanO2-PCMVmin

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2406-Tier1-PhCMV-NTEV

    Plasmid

    #169501

    Purpose

    Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven N-terminal fragment of split tobacco etch virus protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-null (BPK1077)

    Plasmid

    #171128

    Purpose

    Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV and T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJS263

    Plasmid

    #134141

    Purpose

    Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    Ska1 MTBD-His (Y106G6H.15 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)/AAA-nLuc-3XFLAG

    Plasmid

    #127306

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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