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Plasmid#131739PurposeTwo Color Control Assay bicistronic BFP-mCherry-sTRSVctlDepositorInsertsTRSV
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP618
Plasmid#169235PurposeSpear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adaptersDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG
Plasmid#158127PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RNF169
Plasmid#119004PurposeMammalian expression of human RNF169 and BFPDepositorInsertRNF169 (RNF169 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11429
Plasmid#183205PurposepHR-R(NFKB-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11444
Plasmid#183628PurposepHR-R(AP1-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11445
Plasmid#183629PurposepHR-R(ARE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11443
Plasmid#183627PurposepHR-R(NFAT-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11434
Plasmid#183625PurposepHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11435
Plasmid#183626PurposepHR-R(CRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPREDepositorInsertCas12 Guide (crGFP*)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-TetR-Rad18UBD
Plasmid#119010PurposeMammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expressionDepositorInsertTetR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6Rosa-CAG-Cas9
Plasmid#118998PurposeMammalian expression of Rosa26 sgRNA, Cas9 and BFPDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetR-RNF169UBD
Plasmid#119011PurposeMammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertTetR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Rad18UBD
Plasmid#119005PurposeMammalian expression of the human Rad18 Ubiquitin binding domain and BFPDepositorInsertRad18
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RNF169UBD
Plasmid#119006PurposeMammalian expression of the human RNF169 Ubiquitin binding domain and BFPDepositorInsertRNF169 (RNF169 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY (AAV9)
Viral Prep#45185-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsTagBFP, EYFP, or none.Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only