We narrowed to 159,670 results for: addgene

1. pDB4279

   Plasmid

   #98698

   Purpose

   A control plasmid containing the intact bsdMX marker

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER20-FLAG-hPAX3 (#735)

   Plasmid

   #78336

   Purpose

   Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

   Depositor

   Matthew Alexander, Louis Kunkel

   Insert

   PAX3 (PAX3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   TRE2

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

3. ACE2 g2

   Plasmid

   #153012

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNM3732

   Plasmid

   #153086

   Purpose

   mec-4p GFP-C1 positive control for RMCE integration

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p GFP-C1 tbb-2 3'
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6C1A (MBD2)

   Plasmid

   #119958

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD2 (MBD2 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. USP21 (3MTN)

   Plasmid

   #28112

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP21 (USP21 Human)
   

   Use

   Tags

   His tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   catalytic domain only (amino acid residues 209-56…

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

7. ACE2 g3

   Plasmid

   #153013

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCM130

   Plasmid

   #45828

   Depositor

   Mary Lidstrom

   Article

   Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

   Type

   Empty backbone

   Use

   Low-background broad-host-range promoter-probe ve…

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG306-GPD-empty chr I

   Plasmid

   #41895

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118157

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FGD5

    Plasmid

    #28109

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    FGD5:HPC09X-H08:C212499 (FGD5 Human)
    

    Use

    Tags

    His-tag with TEV site

    Expression

    Bacterial

    Mutation

    contains amino acid residues 889-1304.

    Promoter

    Available since

    March 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pRL488

    Plasmid

    #70263

    Purpose

    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.

    Depositor

    Peter Wolk

    Article

    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.

    Type

    Empty backbone

    Use

    Luciferase; Cyanobacteria expression

    Tags

    luxAB

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPhygCAS9

    Plasmid

    #63555

    Purpose

    Expresses CAS9 in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
    

    Use

    Leishmania expression

    Tags

    flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVT-myc-T58A-S62D

    Plasmid

    #138540

    Purpose

    For making myc-T58A-S62D RNA

    Depositor

    Carmen Williams

    Insert

    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Threonine 58 changed to alanine and serine 62 cha…

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDK12

    Plasmid

    #73258

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Insect

    Mutation

    pfam00069: Pkinase 727-1020

    Promoter

    Polyhedrin

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. GALTA-c001

    Plasmid

    #79645

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GALT (GALT Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pESR1.1.0-gDNA

    Plasmid

    #112433

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

    Depositor

    Kevin White

    Insert

    ESR1 (ESR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-dCas9-DNMT3a-Hygro

    Plasmid

    #104403

    Purpose

    transient expression of dCas9-DNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    DNMT3a (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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