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pMK17-02

Plasmid

#99604

Purpose

Plasmid containing parBp(mut)-m(sf)gfp fusion

Depositor

Morten Kjos, Jan-Willem Veening

Article

van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

Insert

parBp(mut)

Use

Synthetic Biology

Tags

m(sf)gfp

Mutation

internal parS-site mutated

Promoter

PczcD2

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pOSIP-CH (CamR, HK022)

Plasmid

#45980

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pKM492-Myc-His

Plasmid

#109283

Purpose

ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Myc-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pOSIP-TH (TetR, HK022)

Plasmid

#45988

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-His10Smt3-gamma-toxin

Plasmid

#197000

Purpose

Bacterial expression plasmid for gamma toxin tRNA endonuclease

Depositor

Dragony Fu

Article

Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

Insert

Gamma toxin

Tags

His10-Smt3

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Promoter

Plac

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTK24

Plasmid

#37406

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pQUASp-mCD8GFP

Plasmid

#46163

Purpose

Expression of murine CD8GFP in insects

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

mCD8GFP

Expression

Insect

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-EGFP-3'UTR#13

Plasmid

#176338

Purpose

The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#13

Expression

Insect

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pKM490-Clip

Plasmid

#109281

Purpose

ORBIT integrating plasmid for C-terminal tagging with CLIP tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-CLIP tag

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRE57-Ter

Plasmid

#170635

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLacZa-GFP

Plasmid

#86440

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

LacZalpha-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

LacZa-sfGFP

Promoter

Plac

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLN

Plasmid

#40028

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

loxP
Neo

Expression

Bacterial and Mammalian

Promoter

None and SV40

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pGGE1-HTR5p

Plasmid

#210542

Purpose

Cloning A. thaliana HTR5 promoter as GreenGate module E1

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pHTR5

Use

Synthetic Biology

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Pxyl-zapT-mScarlet-I

Plasmid

#207249

Purpose

Integrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoter

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

ZapT-mScarlet-I

Tags

mScarlet-I

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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