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8047_GFP-Kras2B_ires_puro

Plasmid

#64371

Purpose

This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

none

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11017 (pICH47732::NOSp-BAR-NOST)

Plasmid

#51145

Purpose

Confers Basta resistance in planta

Depositor

Jonathan D Jones

Insert

NOSp-BAR-NOST

Available Since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAIP4_4QB4

Plasmid

#234187

Purpose

Heterologous protein expression of thermonuclease (TNase) in Escherichia coli

Depositor

Christopher Bahl

Insert

4QB4

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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BAP1-C91A

Plasmid

#108440

Purpose

Stable expression of BAP1-C91A in mammalian cells.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Mutation

C91A: c.271T>G, c.272G>C

Promoter

CMV

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FRB-MCU-Flag-TetOFF

Plasmid

#185659

Purpose

Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

FRB and Flag

Expression

Mammalian

Promoter

pTight TRE

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmFbxw7(res)

Plasmid

#160106

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2F04

Plasmid

#70901

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mod(mdg4) (mod(mdg4) Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pCVSN2c-MCP-oScarlet-KASH-6xMS2 Barcode Library

Pooled Library

#227672

Purpose

RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of CVS N2c strain G-deleted rabies virus

Depositor

Cathryn Cadwell, Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Expression

Mammalian

Species

Synthetic

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only
Mouse Chromatin Regulator Library

Pooled Library

#200011

Purpose

Knockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.

Depositor

Bradley Bernstein, Gabriel Griffin, Robert Manguso

Article

Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only
EGFP miSFIT Lineage Barcoding Library

Pooled Library

#229136

Purpose

The EGFP_miSFIT_Lineage_BC_lib library is a transcribed lineage barcoding vector library.

Depositor

Peter Zandstra

Article

Michaels et al Cell Syst. 2024 Dec 18;15(12):1245-1263.

Expression

Mammalian

Use

Lentiviral

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only
pET21b-C-MBP

Plasmid

#214304

Purpose

Provide a bacterial expression vector that encodes C-terminus MBP tag

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCambia-loxKan

Plasmid

#199590

Purpose

basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility

Depositor

Andreas Stuermer

Insert

FRT-lox66-NosP-KanR-NosT-lox71-FRT

Use

Cre/Lox

Expression

Plant

Promoter

Nos

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pJK295

Plasmid

#71561

Purpose

Production of Acetobacter aceti 1023 thioredoxin (AaTrx)

Depositor

T. J. Kappock

Article

Starks et al Protein Sci. 2007 Jan;16(1):92-8.

Insert

thioredoxin

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYGA

Plasmid

#217672

Purpose

For expression of SUMO tagged human gamma A crystallin

Depositor

Larry David

Insert

CRYGA (CRYGA Human)

Tags

6XHis and Smt3

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETB BS2mT4L1

Plasmid

#141203

Purpose

Chimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Planchestainer et al Catalysts 2018, 8(1), 40

Insert

BS2m-T4L1

Expression

Bacterial

Mutation

E11Q

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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Toronto KnockOut version 3 library (TKOv3)

Pooled Library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Article

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only
pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-IcaR-e

Plasmid

#51176

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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