Addgene

We narrowed to 4,138 results for: ALK

Showing: 1541 - 1560 of 4138 results

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His-SUMO-hDDI2-D252N_pET11a

Plasmid

#169122

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Use

Tags

Expression

Bacterial

Mutation

D252N

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA

Plasmid

#108730

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA

Plasmid

#108747

Purpose

Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa27

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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LiPOP2

Plasmid

#168266

Purpose

A single-component optogenetic tool for photoswitchable pyroptosis

Depositor

Yubin Zhou

Article

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Insert

mGSDMD(1-276) (Gsdmd Mouse)

Use

Tags

cpLOV2, mCherry

Expression

Mammalian

Mutation

R138A, K146A, R152A, R154A

Promoter

CMV

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA

Plasmid

#108745

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA

Plasmid

#108746

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmReNL

Plasmid

#182970

Purpose

Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmReNL

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

Plasmid

#108748

Purpose

Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Ccdc134-IRES-BFP

Plasmid

#117266

Purpose

Retroviral overexpression of Ccdc134

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Ccdc134 (Ccdc134 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeE

Plasmid

#129120

Purpose

pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

PBAD

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-hIes2

Plasmid

#15355

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

PAP-1 binding protein (INO80B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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His-SUMO-Rad23A_pET11a

Plasmid

#169124

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hRad23A (RAD23A Human)

Use

Tags

Expression

Bacterial

Mutation

wt

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBactin-Halo-Tag

Plasmid

#196898

Purpose

Expression of Halo-Tag. Use as control for Halo-tag fusions

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Halo-Tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-SUMO- flag-hDDI2-D252N_pET11a

Plasmid

#169123

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Use

Tags

Expression

Bacterial

Mutation

D252N

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-flag-hDDI2_pET11a

Plasmid

#169121

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLInChR-mVenus

Plasmid

#119298

Purpose

FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

Depositor

Alla Karpova

Article

Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

Insert

Nx1BTM-FCS-ChR2ET/TC-mVenus

Use

AAV

Tags

mVenus

Expression

Mutation

Promoter

CAG

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

Plasmid

#171587

Purpose

Bacterial expression of NLS-RfxCas13d-NLS 6His

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

MKIEE-NLS-RfxCas13d-NLS-HA-6His

Use

Tags

HA-6HIS and MKIEE

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 HA pMT2

Plasmid

#58729

Purpose

expression of HA tagged rat Alpha2 delta-1 in mamalian cells

Depositor

Annette Dolphin

Article

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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