We narrowed to 3,144 results for: PPL;

1. pSmart-EF1alpha-NlaCas7-p65

   Plasmid

   #207394

   Purpose

   Expresses NlaCas7-p65 activator fusion in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-NlaCas7-p65-bGH polyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLP-sfgfp

   Plasmid

   #209994

   Purpose

   Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPT1

   Plasmid

   #209995

   Purpose

   Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   Terminator (TB1006)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPS1

   Plasmid

   #209992

   Purpose

   Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   RBS (RB0034)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLT237

   Plasmid

   #174300

   Purpose

   Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   PolC homology arm
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Cysteine 669 to Tyrosine

   Promoter

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gly-tRNA-gRNA scaffold for library 2

   Plasmid

   #192365

   Purpose

   tRNA sequence provider (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gly tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Pro-tRNA-gRNA scaffold for library 2

   Plasmid

   #192366

   Purpose

   tRNA sequence provider (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Pro tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gln-tRNA-gRNA scaffold for library 1&2

   Plasmid

   #192367

   Purpose

   tRNA sequence provider (for checkpoint and immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gln tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Gly-tRNA-gRNA scaffold for library 1

   Plasmid

   #192368

   Purpose

   tRNA sequence provider (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gly tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pro-tRNA-gRNA scaffold for library 1

    Plasmid

    #192369

    Purpose

    tRNA sequence provider (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Insert

    Pro tRNA-gRNA scaffold
    

    Use

    For golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_VPR_mcherry

    Plasmid

    #167894

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

14. NAX_CAGGS_CAS9_m4_mcherry

    Plasmid

    #167870

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

15. pET3a-FruK

    Plasmid

    #186256

    Purpose

    Overexpression of FruK for purification

    Depositor

    Liskin Swint-Kruse, Emile Van Schaftingen

    Article

    Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

    Insert

    1-phosphofructokinase (fruK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB700 lenti gRNA Hygro_zhang2.0

    Plasmid

    #167915

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB700 lenti gRNA EYFP_zhang2.0

    Plasmid

    #167914

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAX_CAGGS_CAS9_VPR_KanR neo

    Plasmid

    #167893

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NAX_CAGGS_CAS9_VPR_iRFP713

    Plasmid

    #167892

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAX_CAGGS_CAS9_VPR_Hygro

    Plasmid

    #167891

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge