We narrowed to 4,698 results for: pcr

1. pPOTv6-blast-3Ty:syPher2:3Ty

   Plasmid

   #179805

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean, Keith Gull

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   syPher2
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPOTv6-blast-3Ty:pHlourin2:3Ty

   Plasmid

   #179806

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean, Keith Gull

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   pHlourin2
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPOTv7-blast-lssmOrange

   Plasmid

   #179795

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean, Keith Gull

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   lss mOrange
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOTv6-blast-3Ty:mNG:3Ty

   Plasmid

   #179783

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean, Keith Gull

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mNG
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L0-I26 pUBQ10 GG0-GG1

   Plasmid

   #195902

   Purpose

   Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   pUBQ10 (UBQ10 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hp-50%GC-Left-half-120bp

   Plasmid

   #169204

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   50%GC-Left-half-120bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hp-50%GC-Right-half-120bp

   Plasmid

   #169205

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   50%GC-Right-half-120bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hp-50%GC-40bp-Revbindsite

   Plasmid

   #169198

   Purpose

   Force-activating hairpin for 5'-to-3' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   50%GC-40bp-hairpin-binding-site-reversed
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p-sfGFP-pA-FRT-Amp-FRT

   Plasmid

   #176620

   Purpose

   for generating PCR templates with sfGFP and Amp for BAC recombination

   Depositor

   Nathan Lawson

   Article

   Shih et al Development. 2021 Nov 9. pii: 273393. do

   Insert

   super folder green fluorescent protein with FRT-flanked ampicillin cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   H217R - please see dep. comments

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-kcnj20

    Plasmid

    #173150

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj20 (si:ch211-113j13.2 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221-kcnj12b

    Plasmid

    #173142

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj12b (kcnj12b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-kcnj14

    Plasmid

    #173144

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj14 (kcnj14 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-kcnj19a

    Plasmid

    #173148

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj19a (kcnj19a Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221-kcnj19b

    Plasmid

    #173149

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj19b (kcnj19b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-kcnj21

    Plasmid

    #173151

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj21 (zgc:162160 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-kcnj2a

    Plasmid

    #173127

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj2a (kcnj2a Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-kcnj2b

    Plasmid

    #173128

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj2b (kcnj2b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-kcnj3b

    Plasmid

    #173130

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj3b (kcnj3b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-kcnj4

    Plasmid

    #173131

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj4 (kcnj4 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-kcnj9

    Plasmid

    #173135

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj9 (kcnj9 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge