We narrowed to 2,610 results for: ubiquitin

1. pcDNA3.1-N-Myc_535-775 USP7

   Plasmid

   #131247

   Purpose

   Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-N-Myc_DW164AA USP7

   Plasmid

   #131252

   Purpose

   Mammalian expression of N-terminally Myc-tagged DW164AA USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 aspa…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-N-Myc_DED/AAA USP7

   Plasmid

   #131253

   Purpose

   Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert USP7 aspart…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-eGFP-NLS-RNF168

   Plasmid

   #133977

   Purpose

   mammalian expression vector of eGFP tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCMV 3xFLAG-LC3B Q116P G120

   Plasmid

   #129289

   Purpose

   Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

   Plasmid

   #60814

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   eGFP-NLS (SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. GFP-ataxin-3 C14A

   Plasmid

   #89977

   Purpose

   Expresses catalytic inactive GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 14 to Alanine

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

   Plasmid

   #55614

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   C4762 mutated to serine to render HERC2 catalytic…

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

   Plasmid

   #208047

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Expression

   Mutation

   Cys>Ala mutations in conserved RING domain

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)

    Plasmid

    #119315

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 366-852

    Promoter

    CMV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINK-OTUD4 (OTU, aa 22-196)

    Plasmid

    #61413

    Purpose

    Expresses human OTUD4 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-21 and aa 197-1114.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCVpuro-6xHis_SUMO2

    Plasmid

    #164938

    Purpose

    Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Small Ubiquitin Like Modifier 2 (SUMO2 Human)
    

    Use

    Retroviral

    Tags

    6XHIS

    Expression

    Mutation

    Promoter

    PGK promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-USPL1-C236A-pcDNA3.1

    Plasmid

    #85764

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236A

    Promoter

    CMV

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. USPL1cat-C236S-pEYFP_C1

    Plasmid

    #85767

    Purpose

    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    catalytic domain (aa 212-514), catalytic inactiv…

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV10-Flag-Ovtm

    Plasmid

    #51888

    Purpose

    expression vectore in mammalian cells

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mFbxl3 (Fbxl3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ovtm (I364T)

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

    Plasmid

    #119319

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP III (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-84

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-ataxin-3 N-t

    Plasmid

    #89980

    Purpose

    Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    construct contains aa 1-182; deleted aa 183-361

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA2-Cul4A-deltaROC

    Plasmid

    #20713

    Depositor

    Yue Xiong

    Article

    McCall et al Mol Cell Biol. 2008 Sep . 28(18):5621-33

    Insert

    Cullin 4A (CUL4A Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Deletion of residues 594 to 612 from N-terminus

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only