We narrowed to 30 results for: ubiquitin

...The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different...requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The...different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our...

...protein-of-interest and the CRBN E3 ligase complex leading to ubiquitination and proteasomal degradation. Adapted from Nabet...interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in question, it ...endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when auxin is added in...TIR1 F-box associated E3 ligase complex will ubiquitinate the AID-tagged protein which will target it ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

...In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with...have this response, they do contain the specific ubiquitin ligase pathway needed for AID. By tagging proteins...cells, which interacts with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate...

...By Mike Lacy Several types of drugs target the ubiquitin E3 ligase cereblon (CRBN), including popular new...with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted...structure-guided drug design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid...

...Ring finger and WD repeat domain 2 (RFWD2); E3 ubiquitin protein ligase cytoC Cytochrome c DR5 Tumor necrosis...family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known ...CHY zinc finger domain containing 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog... SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1...

...endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin ligase complex. In the presence of auxin, this...developed a chemogenetic system that brings Cas9 to ubiquitin ligase for degradation.      New from the viral...

...an E3 ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits ubiquitin and ...

...degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We...formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

...plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR...

...expression mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  As the name implies, this...General expression mRNA Plant promoter from maize ubiquitin gene Constitutive  Gives high expression in plants...

...Expression collection page Plant CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA...with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation...

...wanted to find a lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting with...

...requirement for transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban...

...labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile...

...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding ...

...ligand binding assays, phage display assays, ubiquitination activity assays, toxin activity, small molecule...

...YFP. After crossing onto a tamoxifen-inducible ubiquitin background, each cell in the mouse that expresses...

...chemokines; enzymes like kinases, phosphatases, and ubiquitin ligases; and many more. This process, however,...

...phosphorylation, acetylation, methylation and ubiquitination. For example, to test if a protein is phosphorylated...

...The authors investigated the role of cytosolic ubiquitin ligases in the degradation of CFTR, specifically...