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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 29 results for: ubiquitin

Showing: 1 - 20 of 29 results

  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different...requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The...different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...protein-of-interest and the CRBN E3 ligase complex leading to ubiquitination and proteasomal degradation. Adapted from Nabet...interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in question, it ...endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when auxin is added in...TIR1 F-box associated E3 ligase complex will ubiquitinate the AID-tagged protein which will target it ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...By Mike Lacy Several types of drugs target the ubiquitin E3 ligase cereblon (CRBN), including popular new...with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted...structure-guided drug design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin ligase complex. In the presence of auxin, this...developed a chemogenetic system that brings Cas9 to ubiquitin ligase for degradation.      New from the viral...

  7. dTAG - You're it!

    Type
    Blog Post
    ...degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We...formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  As the name implies, this...General expression mRNA Plant promoter from maize ubiquitin gene Constitutive  Gives high expression in plants...

  10. p53 Pathway

    Type
    Collection
    ...Ring finger and WD repeat domain 2 (RFWD2); E3 ubiquitin protein ligase cytoC Cytochrome c DR5 Tumor necrosis...family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known ...CHY zinc finger domain containing 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog... SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1...

  14. Plasmids 101: Protein tags

    Type
    Blog Post
    ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding ...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Expression collection page Plant CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA...with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation...

  19. CRISPR Plasmids - Plants

    Type
    Collection
    ...pyogenes Hyg Chen 86196 pYPQ141-ZmUbi-RZ-As Maize ubiquitin 1 none As Cpf1 Qi 91715 pKEE401 yes, cut S. pyogenes...
Showing: 1 - 20 of 29 results