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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 20 results for: ubiquitin

Showing: 1 - 20 of 20 results

  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different...requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The...different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...protein-of-interest and the CRBN E3 ligase complex leading to ubiquitination and proteasomal degradation. Adapted from Nabet...interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in question, it ...endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when auxin is added in...TIR1 F-box associated E3 ligase complex will ubiquitinate the AID-tagged protein which will target it ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...By Mike Lacy Several types of drugs target the ubiquitin E3 ligase cereblon (CRBN), including popular new...with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted...structure-guided drug design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin ligase complex. In the presence of auxin, this...developed a chemogenetic system that brings Cas9 to ubiquitin ligase for degradation.      New from the viral...

  7. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We...formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

  8. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...expression mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  As the name implies, this...General expression mRNA Plant promoter from maize ubiquitin gene Constitutive  Gives high expression in plants...

  12. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding ...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...chemokines; enzymes like kinases, phosphatases, and ubiquitin ligases; and many more. This process, however,...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...AID tag could be targeted for degredation via ubiquitination. The key feature of auxin-induced degredation...
Showing: 1 - 20 of 20 results