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pRS305-Leu2-PINO4-PfV-GS-Sapphire

Plasmid

#116930

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Yeast

Promoter

INO4

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTW040

Plasmid

#64769

Purpose

For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Publication

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Promoter

TDH3

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#78231

Purpose

Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Cas9

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pADH99

Plasmid

#90979

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

Depositor

Aaron Hernday

Publication

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

US_HIS1
FRT
pENO1
C. albicans Cas9
pMAL2
FLP
NAT 1 of 2

Use

CRISPR

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETcon_SARS-CoV-2_RBD

Plasmid

#166782

Purpose

Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

Depositor

Jesse Bloom

Publication

Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

Insert

Spike receptor binding domain (RBD) from SARS-CoV-2 (S )

Expression

Yeast

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAG416-Gal-PR50

Plasmid

#84901

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-PfV-GS-Sapphire

Plasmid

#116932

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Yeast

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDownstream1Lox

Plasmid

#164574

Purpose

Integration of 1 LoxN site in the genome of P. berghei

Depositor

Olivier Silvie

Type

Empty backbone

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mSwAP-In_MC2v2(Rosa26)_Acceptor (pKBA204)

Plasmid

#254036

Purpose

A derivative of pKBA135 with Rosa26-targeting homology arms

Depositor

Jef Boeke

Publication

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

mNeonGree-P2A-FCU1-T2A-NeoR

Use

Synthetic Biology; Mouse targeting

Expression

Mammalian

Promoter

human EF1a

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pRS305-Leu2-PINO4-PfV-GS-Sapphire

Plasmid

#116930

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Yeast

Promoter

INO4

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETcon_SARS-CoV-2_RBD

Plasmid

#166782

Purpose

Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

Depositor

Jesse Bloom

Publication

Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

Insert

Spike receptor binding domain (RBD) from SARS-CoV-2 (S )

Expression

Yeast

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Publication

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Publication

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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Anti-GCN4 [IPI-C11L34] (Recombinant Antibody)

Antibody

#218104-rAb

Purpose

Anti-GCN4 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to HLENEVARLKK sequence.

Depositor

Institute for Protein Innovation (IPI)

Publication

Zahnd et al J Biol Chem. 2004 Apr 30;279(18):18870-7

Recommended applications

Western Blot

Reactivity

Budding yeast

Source species

Rabbit

Isotype

IgG

Trial size

Not available to purchase

Available since

May 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits
pH-tdGFP

Plasmid

#74322

Purpose

pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

Depositor

Joerg Stelling

Publication

Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

Insert

pH-tdGFP

Use

Yeast tagging

Tags

none

Expression

Yeast

Mutation

N149Y, Q204H

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pH-tdGFP

Plasmid

#74322

Purpose

pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

Depositor

Joerg Stelling

Publication

Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

Insert

pH-tdGFP

Use

Yeast tagging

Tags

none

Expression

Yeast

Mutation

N149Y, Q204H

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pYD1-mSA

Plasmid

#39865

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

Aga2, FLAG, and HA

Expression

Yeast

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pYD1-mSA

Plasmid

#39865

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

Aga2, FLAG, and HA

Expression

Yeast

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFA6a-HaloTag-KanMX6

Plasmid

#87029

Purpose

Tag S. pombe genes in C terminal with HaloTag

Depositor

Julien Berro

Insert

HaloTag

Expression

Bacterial and Yeast

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-HaloTag-KanMX6

Plasmid

#87029

Purpose

Tag S. pombe genes in C terminal with HaloTag

Depositor

Julien Berro

Insert

HaloTag

Expression

Bacterial and Yeast

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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