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Plasmid#225312PurposeExpresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsert(–)-Eldh1 (Elen_0511 )
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)
Plasmid#225298PurposeExpresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertCadh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN25-Pct5-(–)-Eddh(Gp3C)
Plasmid#233877PurposeExpresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(–)-Eddh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMR-A-3-Sasg
Plasmid#214349PurposeExpresses T7-RNAP, and cloning backbone for sgRNADepositorInsertT7-RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP1H-mCf
Plasmid#229984PurposeLIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2H-mCf
Plasmid#229985PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP3H-mCf
Plasmid#229987PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 WTSLFD
Plasmid#231881PurposeBacterial expression of N-terminally 6His tagged Gcn4 WTSLFDDepositorInsertGcn4 WTSLFD
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoED W
Plasmid#231871PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoED WDepositorInsertGcn4 ILVtoED W
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoAS
Plasmid#231859PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoASDepositorInsertGcn4 ILVtoAS
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationL123S, I128S, V130A, V135A, L137S, I142SPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 ILVtoED
Plasmid#231857PurposeBacterial expression of N-terminally 6His tagged Gcn4 ILVtoEDDepositorInsertGcn4 ILVtoED
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationL123D, I128E, V130E, V135E, L137D, I142DPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-T4gp47
Plasmid#228212PurposeExpression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonucleaseDepositorInsert47 (47 Escherichia phage T4)
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA016
Plasmid#228231PurposeLevel 0 for 5xOp (GreenGate A module)DepositorInsert5xOp
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BirA-HA3-HIS3MX6
Plasmid#227975PurposeTo insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertBirA
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FRB-HA3
Plasmid#228008PurposeExpresses TurboID-FRB fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterCUP1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FRB-NLS-HA3
Plasmid#228009PurposeExpresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterCUP1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FKBP-HA3
Plasmid#228010PurposeExpresses TurboID-FKBP fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterCUP1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p- TID-FKBP-NLS-HA3
Plasmid#228011PurposeExpresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterCUP1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p-TID-FRB-HA3
Plasmid#228012PurposeExpresses TurboID-FRB fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterCHA1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p-TID-FRB-NLS-HA3
Plasmid#228013PurposeExpresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterCHA1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p- TID-FKBP-NLS-HA3
Plasmid#228015PurposeExpresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterCHA1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BioID2-HA3-HIS3MX6
Plasmid#227977PurposeTo insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertBioID3
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-BASU-HA3-HIS3MX
Plasmid#227979PurposeTo insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX markerDepositorInsertBASU
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-TID-FRB-HA3
Plasmid#227996PurposeExpresses TurboID-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-3HA-KanMX6
Plasmid#228020PurposeFor TurboID-FRB integration; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-3HA-HIS3MX6
Plasmid#228021PurposeFor TurboID-FRB integration; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-HIS3MX6
Plasmid#228023PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-HIS3MX6
Plasmid#228025PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-HISMX6
Plasmid#228027PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FRB-HA3
Plasmid#227984PurposeExpresses BirA-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FKBP-HA3
Plasmid#227986PurposeExpresses BirA-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FRB-NLS-HA3
Plasmid#227985PurposeExpresses BirA-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FKBP-NLS-HA3
Plasmid#227987PurposeExpresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FRB-HA3
Plasmid#227988PurposeExpresses BioID2-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FRB-NLS-HA3
Plasmid#227989PurposeExpresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FKBP-HA3
Plasmid#227990PurposeExpresses BioID2-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p- BioID2-FKBP-NLS-HA3
Plasmid#227991PurposeExpresses BioID2-FKBP-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BASU-FRB-HA3
Plasmid#227992PurposeExpresses BASU-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBASU-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p- BASU-FRB-NLS-HA3
Plasmid#227993PurposeExpresses BASU-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBASU-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p- BASU-FKBP-HA3
Plasmid#227994PurposeExpresses BASU-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBASU-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only