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Plasmid#101166PurposeE. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHD
Plasmid#64033PurposeMammalian expression of TTF-1 homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
EHD2-mEGFP
Plasmid#45932Purposecaveolar protein, confines caveolae to the plasma membraneDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmonomeric EGFP_FrameN1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
EHD2-mCherry
Plasmid#45933Purposecaveolar protein, confines caveolae to the plasma membraneDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
EHD2-T72A-mEGFP
Plasmid#45934Purposedominant-negative EHD2 mutantDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmEGFPExpressionMammalianMutationT 72 changed to A (T72A)PromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
EHD2-T72A-mCherry
Plasmid#45935Purposedominant-negative EHD2 mutantDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmCherryExpressionMammalianMutationT 72 changed to A (T72A)PromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPDm
Plasmid#64040PurposeMammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147, with aa 30 to 32 changed…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHDm
Plasmid#64038PurposeMammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226, with aa 48–50 replaced b…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
UseTagsExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:GUS
Plasmid#108444PurposeArabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertHDT2 promoter (HD2B Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterHDT2Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:HDT2-GFP
Plasmid#108566PurposeArabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in rootsDepositorInsertHDT2 (HD2B Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F22
Plasmid#23080DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysine 5 to Arginine, H4 K5R.PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F24
Plasmid#23081DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysine 16 to Arginine, H4 K16R.PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F25
Plasmid#23082DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed lysine 16 to Glutamine, H4 K16…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F26
Plasmid#23083DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone 4 changed Lysine 16 to glycine, H4 K16G.PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS414-59
Plasmid#23087DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Serine 10 to Alanine, H3 S10A.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJD112
Plasmid#23088DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone 3 Changed Serine 10 to Aspartate, H3 S10D.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJD113
Plasmid#23089DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Serine 10 to Glutamamte, H3 S1…PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only