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POC1541

Plasmid

#226482

Purpose

pMOLC360_VR_I7 (Chloramphenicol R) containing the insert I7 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1542

Plasmid

#226483

Purpose

pMOLC360_VL_I8 (Chloramphenicol R) containing the insert I8 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1543

Plasmid

#226484

Purpose

pMOLC360_VR_I9 (Chloramphenicol R) containing the insert I9 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1544

Plasmid

#226485

Purpose

pMOLC360_VL_I10 (Chloramphenicol R) containing the insert I10 to be assembled in POC1520 (pMOBK360_VR) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1545

Plasmid

#226486

Purpose

pMOLC360_VR_I11 (Chloramphenicol R) containing the insert I11 to be assembled in POC1520 (pMOBK360_VR) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

Plasmid

#232936

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Podxl homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB602-MpPHOT-3xFLAG

Plasmid

#228474

Purpose

MpPHOT-3xFLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT-3xFLAG

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa50H9

Plasmid

#179628

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa50H9

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa3G5

Plasmid

#179629

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa3G5

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa30F3

Plasmid

#179620

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa30F3

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa32G3

Plasmid

#179621

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa32G3

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAb132A11

Plasmid

#179622

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAb132A11

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa45C8S

Plasmid

#179623

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa45C8S

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa2F4

Plasmid

#179624

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa2F4

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge