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...see these vectors, visit Addgene’s website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos...example, the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed...Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences that match the pattern AA(N ...N 19 ). If no suitable match is found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine...

...-Bsa4 as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors...transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning vectors...

...adeno-associated virus (AAV) preps for use in your research.... as an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient...

.... From a single copy of DNA (the template), a researcher can create thousands of identical copies using...synthesizes and ships them as a lyophilized powder. The researcher then needs to reconstitute their primers in liquid...

... is mis-called? Open the trace file and use the search feature in the program to locate the incorrect ...

...Welch to discuss her career path, differences in research culture between the US and Australia, and advice...

...antibiotic resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid...

...Introduction Microscopes are emblematic of biological research and are found in many different types of laboratories...

...while the population expands. At this time, some researchers reduce the concentration of the antibiotic in...

...antibiotic resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid...

...reverse-complement using existing software (a quick internet search will lead you to (Link opens in a new window) here...

...transgene could be lost through recombination. Some researchers choose to maintain cells in selective medium ...

...Plasmid Collections KLF Plasmids Plasmids for KLF Research KLF Depositing Labs Juan Belmonte Linzhao Cheng...

...materials useful for neurodegeneration research.... Neurodegeneration Research Collection Addgene has assembled...collections that may help with your neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools ...studies (GWAS) or other large scale studies. Researchers are now investigating the role that these additional...additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection A collection...monoclonal antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing ...emphasizes their rapid and broad dissemination to the research community. This collection highlights plasmids...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You May Also ...Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional...immune response. Browse Plasmids for Immunology Research Genes have been grouped into tables based on the...immunological assay data for translational and clinical research. Sci Data. Feb 27;5:180015. doi: 10.1038/sdata...

...Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research Reprogramming Plasmids Differentiation...plasmids that have been used in Stem Cell research. You can search or sort this table based on plasmid name...Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells are proliferative, unspecialized...have been studied for over 30 years. All of this research led to Kazutoshi Takahashi and Shinya Yamanaka...helping readers choose a strategy suited to their research. Once you know which method you would like to ...create iPSCs or another cell type. You can also search the iPSC Neurodegenerative Disease Initiative (...browse Addgene’s entire collection of Stem Cell Research Plasmids . For more information on stem cells,...

... Special Collections Cancer Research Cancer Research Plasmids...cancer research, organized by signaling pathway. Special...plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR mTOR is a protein kinase...Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection ...gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from...

...both the design and functionality of searches. A better way to search First, check out the new Addgene homepage... it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid search results: ...specific search terms used, so you will only be shown filters relevant to your current search. We’ll take...dropdown list: The search bar will suggest direct links to other types of searches including depositing...plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched search engine...created can narrow your search results to exactly what you need: Better searching with filters Not every...isn’t new to Addgene’s plasmid search. The new design of the plasmid search page moves the filters from ...

...the opinions or research on each topic. Resources for marching and advocacy If the march for science rallies...the March for Science has the details for the march in Washington as well as the satellite marches. Anyone...the March is not about Democrats or Republicans (16). This is a Pro-Science and Pro-Evidence march. Who...Who is marching and why? Who? All sorts of people are choosing to march on April 22nd. As of April 13th...satellite marches are scheduled to take place around the world. There is no requirement that marchers or supporters...scientists and non-scientists alike are marching for. I will be marching to advocate for science education ...modern research, more funding is required. This is by no means an exhaustive list and every marcher is not...

...Uncovering Ancestral Bias in CRISPR Research: We spoke to researchers from the Boehm and Beroukhim labs ...Week, we're highlighting eight amazing posts on research done in our depositor labs. Some are written by...AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent...tool to try in your own work!) If you've got a research story you want to share on our blog, please reach...