Addgene

We narrowed to 5,144 results for: PEP

Showing: 1581 - 1600 of 5144 results

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pBEL3010

Plasmid

#222984

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_070950 fused to GFP in mammalian cells

Depositor

Gira Bhabha, Damian Ekiert

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBEL3011

Plasmid

#222985

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_070940 fused to GFP in mammalian cells

Depositor

Gira Bhabha, Damian Ekiert

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBEL3012

Plasmid

#222986

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_061510 fused to GFP in mammalian cells

Depositor

Gira Bhabha, Damian Ekiert

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus-apCC-Di

Plasmid

#190711

Purpose

Encodes C-Venus followed by the antiparallel coiled-coil apCC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus-apCC-Di

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus

Plasmid

#190713

Purpose

Control plasmid encoding C-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-apCC-Di-C-Venus

Plasmid

#190709

Purpose

Encodes the antiparallel coiled-coil apCC-Di followed by C-Venus.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

apCC-Di-C-Venus

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTorD-H6

Plasmid

#169039

Purpose

Escherichia coli TorD with C-terminal 6xHis in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

TorD-H6

Use

Tags

6xHis

Expression

Bacterial

Mutation

The mutation N at position 46 was changed back to…

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Ile6Met

Plasmid

#128687

Purpose

Expresses GB1 Ile6Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val29Met

Plasmid

#128688

Purpose

Expresses GB1 Val29Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Ala34Met

Plasmid

#128689

Purpose

Expresses GB1 Ala34Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-TEV-GB1 Val39Met

Plasmid

#128690

Purpose

Expresses GB1 Val39Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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His-TEV-GB1 Val54Met

Plasmid

#128691

Purpose

Expresses GB1 Val54Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pKB237.1

Plasmid

#170031

Purpose

Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL19 Δ alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pKB235.2

Plasmid

#170025

Purpose

Encodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL20 alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pKB237.2

Plasmid

#170032

Purpose

Encodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL19 alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-SRS-FLAG:HIS3MX6

Plasmid

#134447

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-SRS-FLAG:kanMX6

Plasmid

#134446

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFD-AI(TVMV)

Plasmid

#137111

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(TVMV)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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