We narrowed to 12,497 results for: NUC

1. LBR pEGFP-N2 (646)

   Plasmid

   #61996

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LBR (LBR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-NUP188

   Plasmid

   #159893

   Purpose

   Expresses NUP188 in mammalian cells

   Depositor

   Kozo Tanaka

   Article

   Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

   Insert

   NUP188 (NUP188 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   To avoid NotI site, first three amino acids are d…

   Promoter

   CMV

   Available since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

   Plasmid

   #86674

   Purpose

   Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   GFP-FLAG-cGAS E225A/D227A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDESTmycDICER

   Plasmid

   #19873

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   dicer 1, ribonuclease type III (DICER1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCKC014

   Plasmid

   #226623

   Purpose

   pLenti wt hTdT template for library 3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   Terminal deoxynucleotidyl transferase (DNTT Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICS NU-MT P2A

   Plasmid

   #210129

   Purpose

   Detect the Nucleus-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

   Insert

   SPLICS NU-MT P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. px551-mSncg-0.27kb-HA-SpCas9

   Plasmid

   #153208

   Purpose

   Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated mSncg (0.27kb)

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM-AAV-mSncg-Cre

   Plasmid

   #153207

   Purpose

   Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mSncg

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT/TO/FLAG/HA-DEST TNRC6B

    Plasmid

    #19884

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    trinucleotide repeat containing 6B (TNRC6B Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

    Plasmid

    #62044

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LAP2beta (Tmpo Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. NKP90 RFP (787)

    Plasmid

    #62001

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NKP90 (TMEM126A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    pgk

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-sACt-mCherry-NES

    Plasmid

    #138215

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NES (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid

    #160140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(nucleoplasmin)-3xHA-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-NFIB-T2A-miRFP670

    Plasmid

    #187222

    Purpose

    Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Nuclear Factor I B (NFIB Human)
    

    Use

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPlacPbuCas13b-gRNA-T-MS2

    Plasmid

    #184839

    Purpose

    Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and lac promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ΔN-RNMT

    Plasmid

    #112708

    Purpose

    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    FLAG and HIV Rev nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-120, mutated active-site D2…

    Promoter

    CMV

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE4254

    Plasmid

    #89050

    Purpose

    Expresses St chimeric gRNA and human codon-optimized StCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    St chimeric gRNA
    hStCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV asyn S87A

    Plasmid

    #36056

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87A (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S87A

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only