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Plasmid#61990Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET37 (KIAA1161 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
NET97 pmRFP-N2 (641)
Plasmid#61994Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertAADACL1 (NCEH1 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87E
Plasmid#36057DepositorInsertalpha-synuclein S87E (SNCA Human)
UseAAVTagsExpressionMammalianMutationS87EPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
NET37 pmRFP-N2 (450)
Plasmid#61980Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET37 (KIAA1161 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET50 pmRFP-N2 (489)
Plasmid#61983Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET50 (DHRS7 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
STT3A pmRFP (663)
Plasmid#61997Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertSTT3A (STT3A Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygro-Flag-ZO-2-delta-N
Plasmid#27476DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeletion of coding nucleotides from 1-351. This r…PromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 N-T2A-M-IRES-E
Plasmid#231903PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' systemDepositorUseTagsExpressionMammalianMutationR203M in N proteinPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS
Plasmid#228400PurposeEncodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasmDepositorInsertNES/PKI-mNeonGreen2x-NLS/FUS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro
Plasmid#228398PurposeEncodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasmDepositorInsertNLS/cMyc-AcGFP2x-NES/iKB2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro
Plasmid#228399PurposeEncodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasmDepositorInsert3xFLAG-NLS/SV40-AcGFP-NES/PKI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCKC014
Plasmid#226623PurposepLenti wt hTdT template for library 3DepositorInsertTerminal deoxynucleotidyl transferase (DNTT Human)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG071
Plasmid#214257PurposeHybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920DepositorInsertHybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M
UseTagsExpressionBacterialMutationHybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…PromoteraraBAD promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG075
Plasmid#214258PurposeHybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105DepositorInsertHybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M
UseTagsExpressionBacterialMutationHybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…PromoteraraBAD promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP-SUN1ΔN
Plasmid#213516PurposeExpresses human KU70-eGFP-SUN1ΔN in mammalian cellsDepositorInsertKU70-eGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTdelta5cys
Plasmid#207465PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variantDepositorInsertTdTdelta5cys (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENeCFPCNAL2 pc922
Plasmid#167563PurposeFor construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only