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We narrowed to 417 results for: NUC

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  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...MEDLINE-indexed journal Nuclear Receptor Signaling. Dr. McKenna edits the website of the Nuclear Receptor Signaling... elicit their actions on other tissues. At the Nuclear Receptor Signaling Atlas (NURSA), one of our primary...regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators, and their ...their physiological endocrine ligands. Nuclear receptors and their ligands NRs represent the largest family...Consortium represents a unique funding model in nuclear receptor research, and has generated a set of comprehensive...address many important and fundamental questions in nuclear receptor and coregulator research. Our Q-PCR datasets...for example, document the expression patterns of nuclear receptors across multiple different tissues in ...

  2. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... 4D nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...loci, a major benchmark in characterizing the 4D nucleome. Future modifications and applications Ma et al...technique. The NIH Common Fund has made mapping the 4D nucleome a specific priority, as researchers work to increase... nucleome...

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...reagents in a technique known as nucleofection, which forms pores in the nuclear membrane, allowing for entry...translation delays editing What are Cas9-gRNA ribonucleoproteins? One alternative approach, which avoids many...these complications, is to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex...use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step in this type of CRISPR experiment...Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An advantage of performing CRISPR experiments...fusion to a fluorescent protein is used to label nucleic acid targets.  While Cas9-gRNA RNPs show promise... efficient mouse genome editing by CRISPR ribonucleoprotein electroporation of zygotes." Journal of Biological...

  4. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...updated April 16, 2020. Sensitive and specific nucleic acid detection is crucial for clinical diagnostics...biotechnological advancements. Many methods of nucleic acid detection however, either lack the sensitivity...sensitivity or the specificity to detect nucleic acids at low concentrations and/or are too expensive, time-consuming..., and inexpensive platforms to reliably detect nucleic acids at the atomolar level. The Zhang lab has...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen...signal that indicates the presence of your targeted nucleic acid. To increase the sensitivity of the assay,... In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction with Cas13 to amplify...

  5. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger Consortium: Nuclease Expression Vectors This...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created...cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian ...in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric... “-” heterodimeric FokI domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068...by Moore et al. PLoS One 2012 and Cade et al., Nucleic Acids Res 2012 ) pCLR2070 (for expression of a ...

  6. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...Considerations when choosing your Cas nuclease When choosing the Cas9 nuclease for your experiments, there are...but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this...determined by the PAM sequence recognized by the nuclease. For the most frequently used Cas9, spCas9 from... Figure 1: The PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s... measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel...measure of on-target activity (right). Cas9 nucleases that exhibit enhanced site accessibility In order...engineered Cas9, SpCas9-NG, which required only the di-nucleotide PAM NG (Nishimasu et al., 2018). The David Liu...

  7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP) delivery Epitope tagging of mammalian...with recombinant Cas9 protein to form the ribonucleoprotein complex (csRNP, Figure 1). We use in-house...cells recover for 2-5 days.Note: We use the Nucleofection method for delivery of csRNP reagents into cells...tagging in mammalian stem cells using Cas9 ribonucleoprotein. Elife 7. PubMed PMID: 29638216. PubMed Central... by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to...Chinese scientists eager to hear current findings in nucleic acid science. I was delighted to see that the majority...plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne Kamens, PhD Thoughts...structure.” That certainly summed up this conference—nucleic acid science still has many surprises for the researchers... nucleic acids...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...two major lobes, called NUC (for nuclease) and REC (for recognition). The NUC lobe contains the DNA scissors... of Cas9’s two major lobes, REC and NUC, and their subdomains. NUC includes the HNH and RuvC catalytic...conformation cleaves both DNA strands via the NUC lobe, as in Figure 6. NUC lobe, green; REC lobe, light blue; guide... domain (CTD) of the protein also belongs to the NUC lobe. Because its primary role is recognizing the...to, and their roles. Lobe Domain Role NUC HNH Cut DNA target strand RuvC Cut DNA ...’s most often shown in an “open” state, with the NUC and REC lobes laying open like a book (Figure 2)....conformation, as in Figure 3. Upon DNA binding, the NUC lobe recognizes the PAM site in the non-target DNA...

  10. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP...pAAV-Ef1a-fDIO mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP...

  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...forebrain GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg)...H134R)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors...switch that expresses nuclear mCherry in the absence of Cre, and expresses nuclear EGFP in the presence...

  13. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...37354 pQC NLS mCherry IX Nucleus NLS mCherry Connie Cepko 14893 pEBFP2-Nuc Nucleus NLS EBFP2 Robert Campbell...14894 pmKalama1-Nuc Nucleus NLS mKalama1 Robert Campbell 189776 pmScarlet3-LaminB_C1 Nuclear envelope LaminB1...p3xnls-mScarlet3_N1 Nucleus 3xNLS mScarlet3 Dorus Gadella 61168 pCMU-NUCr Nucleus (Plants) NLS mCherry...3xnls-mNeonGreen Nucleus 3xNLS mNeonGreen Dorus Gadella 98817 3xnls-mTurquoise2 Nucleus 3xNLS mTurquoise2...pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus...mCherry-LaminA-C-18 Nuclear envelope LaminA1 mCherry* Michael Davidson 98830 mTurquoise2-LaminB Nuclear envelope ...pCMV-mGold2t-CAF1-C-10 Nucleus CAF-1 mGold2t Francois St-Pierre 231774 pCMV-mGold2s-CAF1-C-10 Nucleus CAF-1 mGold2s...

  14. Control AAV Preps

    Type
    Collection
    ...PHP.S Edward Boyden 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre...

  15. Retrograde AAV viral preps

    Type
    Collection
    ...Control Viviana Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Jonathan Ting 51083 AAV-...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Jonathan ...GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Jonathan Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal pGADCg...Guide and the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid...3xHA-tagged TurboID or miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells...

  17. CRISPR Guide

    Type
    Collection
    ...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity...break; the wild type function of most Cas nucleases dCas9 Nuclease dead Cas9, an enzymatically inactive form...required scientists to design and generate a new nuclease pair for every genomic target. Due to its comparative...RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic ...necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be ...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...proliferator-activated receptor delta FAAR, MGC3931, NR1C2, NUC1, NUCI, NUCII, PPAR-beta, PPARB PPARG peroxisome proliferator-activated...HLALS NFYA nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription...FLJ37489 VAV1 vav 1 guanine nucleotide exchange factor VAV VAV2 vav 2 guanine nucleotide exchange factor - VAV3...NROB1 NR0B2 nuclear receptor subfamily 0, group B, member 2 FLJ17090, SHP, SHP1 NR1D1 nuclear receptor subfamily...hRev NR1D2 nuclear receptor subfamily 1, group D, member 2 BD73, EAR-1R, RVR NR1H2 nuclear receptor subfamily... UNR NR1H3 nuclear receptor subfamily 1, group H, member 3 LXR-a, LXRA, RLD-1 NR1H4 nuclear receptor subfamily...MGC97144, MGC97209 NR2C1 nuclear receptor subfamily 2, group C, member 1 TR2 NR2C2 nuclear receptor subfamily...

  19. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription...Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP ...Cohesins 114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP...transcription factor UBF Nucleolus (fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744...Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20...reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP ...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP...

  20. Open Enzyme Collection

    Type
    Collection
    ...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli E... E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease VIII 165571 pOpen-Exonuclease...pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4...Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda...
Showing: 1 - 20 of 417 results