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Plasmid#205713PurposeMammalian expression of mNeonGreen-alpha-synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PI4KB
Plasmid#221759PurposeStable expression of GFP-PI4KB in mammalian cellsDepositorInsertPI4KB (PI4KB Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1324
Plasmid#217965PurposeBacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.DepositorInsertImp5 (IPO5 Human)
UseTagsGSTExpressionBacterialMutationPromoterplacAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 G224A-mGFP
Plasmid#180819PurposeExpression of human PD-1 G224A fused to mEGFPDepositorInsertPD-1 (PDCD1 Human)
UseLentiviralTagsmEGFPExpressionMutationG224APromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125228PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuclear-EKAR4
Plasmid#174439PurposeNuclear-targeted EKAR4.DepositorInsertnuclear-EKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP
Plasmid#114369Purposeexpresses Flpo recombinase-dependent GtACR2DepositorInsertGtACR2-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry N1 TOMM22
Plasmid#157762PurposeExpression of TOMM22:mcherry in cellsDepositorInsertTOMM22 (TOMM22 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS26_QUAS_CsChrimson
Plasmid#175548PurposeExpresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integrationDepositorInsertCsChrimson
UseTagstdTomatoExpressionInsectMutationPromoterQUASAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)
Plasmid#213402Purposedouple floxed, myristoylation (myr) site for membrane targeting and EGFPDepositorInsertEGFP
UseAAVTagsC-terminal (Ct) cytoplasmic domains of low densit…ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal-BE3
Plasmid#172409PurposeYeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoterDepositorInsertBE3
UseCRISPRTagsExpressionYeastMutationPromoterGALLAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIA22
Plasmid#214448PurposeExpresses BxbI-BP-NLSDepositorInsertBxbI
UseTagsBP-NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-Silent_Mutation_WT
Plasmid#187986Purposehuman p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationsilent mutation for resistance to sip62 #5PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKD2-ChR2-mCherry
Plasmid#170845PurposeAAV vector for Cre-dependent transgene expression of ChR2-mCherry in cortical interneurons under the control of the Dlx enhancer.DepositorInsertChR2-mCherry
UseAAVTagsExpressionMammalianMutationPromoterDlxAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-TVA-IRES-Puro
Plasmid#172366PurposeGeneration of TVA-expressing stable cell lines, resistant to the antibiotic PuromycinDepositorInsertTVA + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8s
Plasmid#172389PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8s
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only