We narrowed to 8,725 results for: Set

1. pFvL118

   Plasmid

   #64752

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW081

   Plasmid

   #64755

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHPK1212

   Plasmid

   #8834

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

   Insert

   tobacco vein mottling virus protease, catalytic domain
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_dNmeCas9

   Plasmid

   #120077

   Purpose

   dNmeCas9 with no sgRNA cassette lenti construct

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   dNmeCas9
   

   Use

   Lentiviral

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3087 pBS Bcl-2-Ig

   Plasmid

   #8809

   Depositor

   Stanley Korsmeyer

   Article

   Seto et al EMBO J 1988 Jan;7(1):123-31.

   Insert

   Bcl-2-Ig (BCL2 Human)
   

   Expression

   Bacterial

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

6. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

   Plasmid

   #242660

   Purpose

   CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette

   Depositor

   Benjamin Kleinstiver

   Insert

   pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
   

   Use

   AAV and CRISPR

   Tags

   BPNLS

   Mutation

   VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

   Promoter

   CBh

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. S111

   Plasmid

   #174549

   Purpose

   Constitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   BclxL and designed protein LD3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. S112

   Plasmid

   #174550

   Purpose

   Constitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   Bcl2 and designed protein LD3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW087

   Plasmid

   #64756

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 His6-mEGFP-D4

    Plasmid

    #226372

    Purpose

    Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4 (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Promoter

    Ptet

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDOC-GG

    Plasmid

    #149377

    Purpose

    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Inserts

    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIP GFP-MOSPD2 WT

    Plasmid

    #186467

    Purpose

    Expression of human MOSPD2 fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIFR-3

    Plasmid

    #233295

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Promoter

    p14G

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATP1A1_T804N_hPGK1_mScarlet-I_Donor

    Plasmid

    #173207

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N mScarlet-I donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hPGK1

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT-GEM(0)

    Plasmid

    #62891

    Purpose

    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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