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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,976 results for: CAN

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Showing: 1641 - 1660 of 13976 results

  6. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  12. pEIAV-DIO-TLoop-CHR2YFP

    Plasmid
    #66798
    Purpose
    expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector
    Depositor
    Edward Callaway
    Article
    Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.
    Insert
    Floxed Chr2-YFP
    Use
    Lentiviral
    Tags
    citrine
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    CMVTetO7 Promoter
    Available Since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    66798-plasmid-map-sequence-id-114340

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  13. pFH6

    Plasmid
    #86555
    Purpose
    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    U6-26p::sgRNA scaffold
    Use
    CRISPR; Subcloning
    Promoter
    Arabidopsis U6-26 promoter
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86555-plasmid-map-sequence-id-176427

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  14. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  19. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54321-plasmid-map-sequence-id-84455

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Showing: 1641 - 1660 of 13976 results