We narrowed to 2,242 results for: D10

1. LAMP1-mCherry-FRB*

   Plasmid

   #207145

   Purpose

   Lysosome-targeted rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Tags

   FRB* (T2098L mutant of FKBP-rapamycin binding dom…

   Expression

   Mammalian

   Mutation

   Silent mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_UdgX-EE-UdgX-nCas9-UdgX

   Plasmid

   #163566

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ-CBE-SpRYn-Act3.0

   Plasmid

   #178958

   Purpose

   It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2022

   Plasmid

   #142905

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NPAS4 (NPAS4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-mA3-BE3

   Plasmid

   #113419

   Purpose

   Expresses mA3-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-ABE-SpRYn-Act3.0

   Plasmid

   #178959

   Purpose

   It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

   Plasmid

   #163543

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hA3G-BE3

   Plasmid

   #113415

   Purpose

   Expresses hA3G-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB80-LAMP1-mCherry-iLID

   Plasmid

   #174625

   Purpose

   Optogenetic coupling to lysosomes (LAMP1) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   LAMP1-mCherry-iLID (LAMP1 Synthetic, Human)
   

   Tags

   mCherry-iLID

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del

   Promoter

   Chicken beta-actin

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-hA3A-BE3-D131Y

    Plasmid

    #113424

    Purpose

    Expresses hA3A-BE3-D131Y in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_D131Y

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-hA3H-BE3

    Plasmid

    #113416

    Purpose

    Expresses hA3H-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

    Plasmid

    #163559

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-TRE3G-BE3RA-PGK-Puro

    Plasmid

    #110846

    Purpose

    Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    TRE3G

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEX-SP-CFP-STIM1(23-685)

    Plasmid

    #18858

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted between residues 1-23 (signal peptid…

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    WT OGT

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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