We narrowed to 4,798 results for: pcr
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pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP
Plasmid#139484PurposePCR template for mEGFP gene knock-in donor vectorDepositorInsertmEGFP-loxP-P2A-dTKneo-pA-loxP
TagsmEGFPExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
Plasmid#86424PurposePCR tagging module with yeast codon optimized mTurquoise2DepositorInsertmTurquoise2
ExpressionYeastAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*
Plasmid#99515PurposeC-terminal AID* degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteMutationinternal inversion of nucleotides 265-312 of the …Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
-2.5Kb MMP10
Plasmid#53973PurposeLuciferase reporter for mouse MMP10 2.5kb promoterDepositorInsertmouse MMP10 2.5Kb promoter (Mmp10 Mouse)
UseLuciferaseExpressionMammalianPromoterPCR from C57BL6/JAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
TagsHaloTagExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)-8myc
Plasmid#99516PurposeTruncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiaeDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11
Plasmid#173139PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11 (kcnj11 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingMutationhuman codon-optimizedAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTGC-U6.2
Plasmid#112809PurposePCR template vector for amplifying gRNAcore-pU6.3 promoter fragmentDepositorInsertU6:2-gRNAcore
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGC
Plasmid#112812PurposePCR template vector for amplifying gRNA(F+E)-OstRNA fragmentDepositorInsertgRNAcore(F+E)-tRNA
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(31-114)-9myc
Plasmid#99517PurposeTruncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiaeDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain
Plasmid#33331DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
TagsFLAGExpressionMammalianMutationCaMKKalpha RP domain (167-189) was replaced with …PromoterSV40Available SinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMB84 (Nf2 C-term)
Plasmid#12169DepositorInsertNf2 C-terminal half (Nf2 Mouse)
UseCre/LoxExpressionMammalianMutationC-terminal half of Nf2 cDNAAvailable SinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pNDGG039
Plasmid#231339PurposeTerminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.DepositorInsertBEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
ExpressionBacterialAvailable SinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only
