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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,802 results for: pcr

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Showing: 1641 - 1660 of 4802 results

  1. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid
    #202199
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Has Service
    AAV1 and AAV5
    Insert
    stChrimsonR, PdCO
    Use
    AAV and Cre/Lox
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Promoter
    hSyn1
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202199-plasmid-map-sequence-id-397078

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  4. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  6. pJL257

    Plasmid
    #243696
    Purpose
    A piggyBac vector containing the NDUFB9 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    UTRs and CDS of NDUFB9 (NDUFB9 Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243696-plasmid-map-sequence-id-489271

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  12. pJL355

    Plasmid
    #243710
    Purpose
    A piggyBac vector containing the endogenous COX7A2 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    COX7A2 genomic locus, with its 3' UTR replaced by the ACTB 3' UTR (COX7A2 Human)
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243710-plasmid-map-sequence-id-489295

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  14. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  19. mTF-CRISPRai

    Pooled Library
    #122131
    Purpose
    Increases transcriptional repression of genes encoding transcription factors in combination with dCas9 repression systems, such as the dCas9-KRAB system.
    Depositor
    Stanley Qi
    Article
    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-
    Expression
    Mammalian
    Species
    Mus musculus
    Use
    CRISPR and Lentiviral
    Available Since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1641 - 1660 of 4802 results