Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,323 results for: Coli

Showing: 3401 - 3440 of 10323 results

Results per page:

Sort results by:

pBSKa2_FRTtetFRT

Plasmid

#243842

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetA

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa1_FRTkanFRT

Plasmid

#243835

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5607

Plasmid

#250513

Purpose

pSecYEG; cell-free expression of functional SecYEG

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-secYEG

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5608

Plasmid

#250514

Purpose

Cell-free expression of SecA motor protein for translocation through SecYEG

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-secA

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pR6Ko1_FRTampFRT

Plasmid

#243898

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pR6Ko1_FRTkanFRT

Plasmid

#243899

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pR6Ko1_FRTspcFRT

Plasmid

#243902

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa1_FRTcamFRT

Plasmid

#243836

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cat

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa1_FRTtetFRT

Plasmid

#243837

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa1_FRTspcFRT

Plasmid

#243838

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa2_FRTampFRT

Plasmid

#243839

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa2_FRTkanFRT

Plasmid

#243840

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa2_FRTcamFRT

Plasmid

#243841

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cat

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa2_FRTspcFRT

Plasmid

#243843

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKa1_FRTampFRT

Plasmid

#243834

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. May be omega-assembled with another transcription unit.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and C55I

Plasmid

#248807

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and C55I

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and R337P

Plasmid

#248805

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and R337P

Plasmid

#248817

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA1370_pTet-ECOR31_Hachiman

Plasmid

#223364

Purpose

Expression of ECOR31 Hachiman under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and C55M

Plasmid

#248819

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and C55M

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and P167I

Plasmid

#248816

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and P167I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and T57I

Plasmid

#248806

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and T57I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and A48R

Plasmid

#248821

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and A48R

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and P167I

Plasmid

#248804

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and P167I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and Y139S

Plasmid

#248810

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and Y139S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and A48R

Plasmid

#248809

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and A48R

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and A48N

Plasmid

#248811

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and A48N

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplA W37V and E265R

Plasmid

#248812

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and E265R

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and T57I

Plasmid

#248818

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and T57I

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and F147S

Plasmid

#248820

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and F147S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and E265R

Plasmid

#248824

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and E265R

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and Y139S

Plasmid

#248822

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and Y139S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and F147S

Plasmid

#248808

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and F147S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and A48N

Plasmid

#248823

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and A48N

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V and L172A

Plasmid

#248815

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and L172A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA W37V

Plasmid

#248814

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-TEV-LplA

Plasmid

#248813

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplA Wild Type

Plasmid

#248801

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-mCherry-Flag-LplAW37V and L172A

Plasmid

#248803

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and L172A

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-Y11

Plasmid

#239000

Purpose

Final circuit with -Ara: ON, +Ara: OFF behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression only in the absence of Ara (-Ara: ON, +Ara: OFF)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3401 - 3440 of 10323 results

Results per page:

Sort results by: