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Showing: 8621 - 8630 of 9134 results

  3. qTAG-KO-Puro-TP53

    Plasmid
    #227319
    Purpose
    Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227319-plasmid-map-sequence-id-451734

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  4. qTAG-C-mStayGold-EFS-Puro-CEP192

    Plasmid
    #227290
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227290-plasmid-map-sequence-id-451300

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  8. AA324

    Plasmid
    #215953
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215953-plasmid-map-sequence-id-431481

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  9. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  10. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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Showing: 8621 - 8630 of 9134 results