We narrowed to 1,247 results for: ncl
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Plasmid#8618DepositorInsertribonuclease Sa3, barstar
TagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…Available SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-mCherry-enCas9(D10A)-PolI5M
Plasmid#249060PurposeExpresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertenCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceDec. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-mCherry-enCas9(D10A)-PolI3M
Plasmid#249061PurposeExpresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertenCas9-PolI3M
UseCRISPRExpressionMammalianAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-dCas9-PolI5M
Plasmid#249065PurposeExpresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertdCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-enCas9(D10A)
Plasmid#249063PurposeExpresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertenCas9
UseCRISPRExpressionMammalianAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Gluc-feRMA-IRES-EGFP
Plasmid#248190PurposeExpresses Gluc-feRMA and EGFP under the hSyn promoter. Gluc-feRMA enables monitoring of neuronal transduction and includes a TEV cs, allowing inactivation of it upon interaction with TEV protease.DepositorInsertsGaussia luciferase with TEV cleavage site fused to IgG1 Fc
IRES-EGFP
UseAAV; LuciferaseTagsGluc (N terminal on insert) and IgG1 Fc (C termin…ExpressionMammalianPromoterIRES and hSynAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-puro-atpG
Plasmid#248067PurposeLentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.DepositorInsertatpG (atpG E. coli)
UseLentiviralTagsFLAGMutationcodon-optimized for expression in human cellsPromoterTRE3GSAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-bsd-atpA
Plasmid#248065PurposeLentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.DepositorInsertatpA
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterTRE3GSAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-CrPV-IRES-cytERM-SUNTAG-MS2
Plasmid#247299PurposeMammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.DepositorInsertPB-CrPV-IRES-cytERM-SUNTAG-MS2
ExpressionMammalianAvailable SinceNov. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry
Plasmid#242649PurposeFloxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.DepositorInsertFloxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
UseAAV, Cre/Lox, and Mouse TargetingExpressionMammalianMutationThe amyloid precursor reading frame contains a c…Promoterhuman synapsinAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only