Addgene

We narrowed to 3,199 results for: eda

Showing: 1721 - 1740 of 3199 results

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pCas9-EcRT-Z3-Hyg

Plasmid

#232095

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Z3

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Nat

Plasmid

#232094

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Z3

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Hyg

Plasmid

#232098

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Kan

Plasmid

#232096

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Z3

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3K253E

Plasmid

#225739

Purpose

To express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in Rv3542c in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3K253E

Use

Tags

c-Myc

Expression

Bacterial

Mutation

igr3 K253E

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3WT

Plasmid

#225738

Purpose

To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3WT

Use

Tags

c-Myc

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HF-CDC50B

Plasmid

#209224

Purpose

Mammalian expression of CDC50B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

CDC50B (TMEM30B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW2-MaUPPS-B

Plasmid

#203175

Purpose

Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

MA_4402

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-MaUPPS-A

Plasmid

#203174

Purpose

Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

uppS

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub5

Plasmid

#201738

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) epsilon-tubulin Tub5 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub5

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub3-mScarlet

Plasmid

#201735

Purpose

Tardigrade (Hypsibius exemplaris) gamma-tubulin Tub3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin gamma

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D4-mScarlet

Plasmid

#201734

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D4 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D4

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D3-mScarlet

Plasmid

#201733

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D3

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D2-mScarlet

Plasmid

#201732

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D2 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D2

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2C-mScarlet

Plasmid

#201731

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2C tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta C

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1C1

Plasmid

#201730

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1C1

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1A2

Plasmid

#201729

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A2 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A2

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1A1

Plasmid

#201728

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A1

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-145-146

Plasmid

#198600

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-145-146

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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