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pBiT3.1-C_GEMIN5:1-1508

Plasmid

#211164

Purpose

Mammalian expression of full-length human GEMIN5. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

Gemin5:M1-M1508 (GEMIN5 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_SCAP:1-905

Plasmid

#211302

Purpose

Mammalian expression of full-length human SCAP isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DCAF11:1-546

Plasmid

#211271

Purpose

Mammalian expression of full-length human DCAF11. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R207H natural variant (VAR_020121)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_DCAF12:1-453

Plasmid

#210966

Purpose

Mammalian expression of full-length human DCAF12. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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FGFR2 F276C 3xFlag

Plasmid

#110064

Purpose

FGFR2 F276C expression in mammalian cells (JCOPO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Phenylalanine 276 to Cysteine (F276C)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_LRRK2:2141-2527

Plasmid

#210899

Purpose

Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:T2141-E2527 (LRRK2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

M2397T natural variant ( VAR_024965)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_GEMIN5:1-739

Plasmid

#211124

Purpose

Mammalian expression of human GEMIN5 fragment M1 to A739. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

Gemin5:M1-A739 (GEMIN5 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_LRRK2:2142-2527

Plasmid

#211112

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

M2397T natural variant (VAR_024965)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_GEMIN5:1-1508

Plasmid

#211123

Purpose

Mammalian expression of full-length human GEMIN5. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

Gemin5:M1-M1508 (GEMIN5 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF12:1-453

Plasmid

#211198

Purpose

Mammalian expression of full-length human DCAF12 N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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