We narrowed to 2,556 results for: EXO

1. pDONOR-PARK6e5-I368N

   Plasmid

   #86154

   Purpose

   Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   PINK1 exon 5 homology arms (PINK1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

   Plasmid

   #99743

   Purpose

   Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Use

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Changed both L165 and E166 to Alanine

   Promoter

   CMV

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

   Plasmid

   #216152

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

   Plasmid

   #216154

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-Htt171-66Q-myc-WPRE

   Plasmid

   #107934

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-Htt171-18Q-myc-WPRE

   Plasmid

   #107936

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of wild type HTT (HTT Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

   Plasmid

   #158542

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Amino acids 1-480, C-terminal deletion, F188D, W2…

   Promoter

   CMV

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSin-EF2-DMD53-GFP

   Plasmid

   #138353

   Purpose

   Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences

   Depositor

   Baisong Lu

   Article

   Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do

   Insert

   DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   EF2

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CuO-V5 CASC3 siRes 110-480 WT

   Plasmid

   #158543

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Amino acids 110-480, C-terminal deletion

   Promoter

   CMV

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11a_3xFlag-nsp10 (SARS-CoV-2)

    Plasmid

    #169157

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEXSISERS_FLuc_loxP-PuroR

    Plasmid

    #177866

    Purpose

    Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    FLuc-based EXSISERS
    

    Use

    CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

    Plasmid

    #161924

    Purpose

    To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting RDH11 exon 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEXSISERS_NLuc_FRT-PuroR

    Plasmid

    #177865

    Purpose

    Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    NLuc-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEXSISERS_BSD_FRT-PuroR

    Plasmid

    #177867

    Purpose

    Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    BSD-based EXSISERS
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEXSISERS_SurfaceHaloTag_FRT-PuroR

    Plasmid

    #177869

    Purpose

    Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    Surface-HaloTag-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169162

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    6His-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRRL-MND-hCard11 delta e4-T2A-GFP

    Plasmid

    #163771

    Purpose

    Expression of CARD domain variant with a deletion of exon 4

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 4

    Promoter

    MND

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRL-MND-hCard11 delta e3-4-T2A-GFP

    Plasmid

    #163772

    Purpose

    Expression of CARD domain variant with a deletion of exons 3 and 4.

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 3-4

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRRL-MND-hCard11 delta e3-T2A-GFP

    Plasmid

    #163770

    Purpose

    Expression of CARD domain variant with a deletion of exon 3

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 3

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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