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Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterT7/LacO promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon
Plasmid#206218PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-Tev
Plasmid#84503PurposeMESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev proteaseDepositorInsertV2-MESA-35F-Tev
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-Tev
Plasmid#84501PurposeMESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev proteaseDepositorInsertV1-MESA-45F-Tev
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast
Plasmid#206217PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEP110E
Plasmid#97396Purposebinary construct of nuclear mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsNucleus targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-M-tTA
Plasmid#84502PurposeMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV2-MESA-35F-M-tTA
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry EF1a
Plasmid#206225PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-tTA
Plasmid#84500PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV1-MESA-45F-M-tTA
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP112E
Plasmid#97398Purposebinary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsMitochondria targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only