We narrowed to 2,202 results for: K-Ras protein

1. pTM1_VP30_ZEBOV

   Plasmid

   #69119

   Purpose

   Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

   Insert

   VP30 ZEBOV (VP30 )
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSplit2-NO-PRPF6

   Plasmid

   #51740

   Purpose

   Express LucN-PRPF6 fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   PRP6 (PRPF6 Human)
   

   Use

   Tags

   FLAG and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSplit-OC-U5-40K

   Plasmid

   #51741

   Purpose

   Express U5-40K-LucC fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   U5-40K (SNRNP40 Human)
   

   Use

   Tags

   FLAG and Luciferase (394-550aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. PA-RL-mCherry

   Plasmid

   #78861

   Purpose

   DULIP negative control (bait) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPA-RL-GW

   Plasmid

   #78855

   Purpose

   DULIP Bait Destination Vector (Gateway, N-terminal)

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Protein A-Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGW-RL-PA

   Plasmid

   #78856

   Purpose

   DULIP Bait Destination Vector (Gateway, C-terminal)

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Renilla luciferase, Protein A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2ADWpuro_2paRAF

   Plasmid

   #129653

   Purpose

   For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2ADW_2paRAF

   Plasmid

   #129654

   Purpose

   For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPA-RL-FL

   Plasmid

   #78863

   Purpose

   DULIP Tandem Construct used for data normalization

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   Firefly
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-4T1 human PP2A Aα full length

    Plasmid

    #132634

    Purpose

    expresses human PP2A Aα full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A Aα (PPP2R1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T1 human PP2A B56α full length

    Plasmid

    #132635

    Purpose

    expresses human PP2A B56α full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A B56α
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Sfp R4-4 mutant

    Plasmid

    #173491

    Purpose

    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

    Depositor

    Jun Yin

    Article

    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

    Insert

    Sfp-R44
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75236

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMIG-hH4-AVITEV

    Plasmid

    #74051

    Purpose

    retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Histone-H4 (H4C16 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75237

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIG-hNFATc1/bC - AVITEV

    Plasmid

    #74059

    Purpose

    retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform beta-C (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV40-EF1a-hBCL10-SO

    Plasmid

    #75256

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs, EF1a

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-FLAG-hHelios-IRES-GFP

    Plasmid

    #74047

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Helios (IKZF2) (IKZF2 )
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    encodes shorter version of human Helios

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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