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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,477 results for: pcr

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Showing: 1761 - 1780 of 6477 results

  11. pJJS003

    Plasmid
    #188326
    Purpose
    PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Publication
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::mCherry::Linker
    Expression
    Bacterial
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188326-plasmid-map-sequence-id-370752

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  13. pECT1

    Plasmid
    #75446
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Publication
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Expression
    Bacterial
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75446-plasmid-map-sequence-id-146474

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  15. p5E-2.3k-fabp2

    Plasmid
    #84927
    Purpose
    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector
    Depositor
    John Rawls
    Insert
    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    Tags
    NO
    Expression
    Bacterial
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    84927-plasmid-map-sequence-id-254043

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  16. OPRD1-Tango

    Plasmid
    #66461
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPRD1 (OPRD1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    66461-plasmid-map-sequence-id-417858

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  18. CHRM1-Tango

    Plasmid
    #66248
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRM1 (CHRM1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  19. pCMV-Sport6-CD9-pHluorin

    Plasmid
    #130905
    Purpose
    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD9-pHluorin (CD9 Aequorea victoria, Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Mutation
    phluorin inserted between Asn-50 and Asn-51 in ex…
    Promoter
    CMV
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    130905-plasmid-map-sequence-id-272542

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  20. pcDNA3.1.Gαq-LgB97

    Plasmid
    #134360
    Purpose
    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαq-LgB97 (GNAQ Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134360-plasmid-map-sequence-id-306401

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Showing: 1761 - 1780 of 6477 results