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Showing: 321 - 360 of 1183 results

  11. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187611
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187611-plasmid-map-sequence-id-372189

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  12. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187612
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain (R163A) with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Arg163Ala
    Promoter
    EF1A
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187612-plasmid-map-sequence-id-372035

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  14. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

    Plasmid
    #148118
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148118-plasmid-map-sequence-id-473831

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  15. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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  17. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Jeremy Wilusz
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    91803-plasmid-map-sequence-id-180321

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  18. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Human, Synthetic)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

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  20. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid
    #64921
    Purpose
    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPE1 (PCBP1 Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64921-plasmid-map-sequence-id-109667

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  21. pAG416GAL-DENV_NS3-GS-NS2B

    Plasmid
    #201940
    Purpose
    Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    DENV NS3-GS-NS2B (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    201940-plasmid-map-sequence-id-402859

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  22. pAG416GAL10-WNV_NS2B-GS-NS3

    Plasmid
    #203475
    Purpose
    Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    WNV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203475-plasmid-map-sequence-id-402805

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  23. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  24. pAG416GAL10-POWV_NS2B-GS-NS3

    Plasmid
    #203471
    Purpose
    Expresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    POWV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203471-plasmid-map-sequence-id-403573

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  25. pAG416GAL10-LGTV_NS2B-GS-NS3

    Plasmid
    #203468
    Purpose
    Expresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    LGTV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203468-plasmid-map-sequence-id-403572

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  26. pAG416GAL10-ZIKV_NS2B-GS-NS3

    Plasmid
    #203477
    Purpose
    Expresses ZIKV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    ZIKV NS2B-GS-NS3 (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203477-plasmid-map-sequence-id-402647

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  27. PARP14 (PARP14A-c013)

    Plasmid
    #110254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP14 (PARP14 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110254-plasmid-map-sequence-id-212336

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  28. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

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Showing: 321 - 360 of 1183 results