...consensus sequence for the addition of a poly(A) tail, the addition of a poly(A) tail on a prokaryotic transcript...regulatory elements, such as the polyadenylation or poly(A) signal. While many studies focus on promoter ...in the RNA transcript followed by a weakly bound poly-uracil tract as shown in the figure to the right...eukaryotic-specific process, prokaryotes also add poly(A) tails to certain RNAs. Unlike the eukaryotic ...added to any accessible 3' end. The presence of the poly(A) tail targets the RNA to the degradosome, which...protected by secondary structure. It is thought that poly(A)s are used to control the cellular concentration...available for polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, ...

...pipette Reagents 1X PBS Microcentrifuge tubes Sterile Poly-D-lysine coated coverslips HeLa cells 24-well plate...Procedure Section 1: Seeding cells Place a sterile poly-D-lysine coated coverslip in each well of a 24-well...

... science policy, there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you...about where science policy happens.  When do you do science policy? Many science policy training programs... is science policy?”  I have been asked this many times throughout my short science policy career and ...importantly, how, of a science policy career.  Who does science policy? I’ve intentionally started with... many science policy professionals that entered the field from a more traditional policy background, learning...to start. What is science policy? THE IMPOSSIBLE QUESTION. Science policy is the work you do to creatively...pressures including economic, cultural, and political. Science policy looks differently depending on how and...

...1: EvolvR consists of an error-prone polymerase (PolI) that is fused to a nicking Cas9. A guide directs...nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the 3' end of the...which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target mutagenesis rate...EvolvR: (1) two additional mutations to PolI3M generated PolI5M which increased EvolvR’s mutagenesis rate...conditions. Expression of an error prone polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this... system has two key components: the error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar...introduce mutations. However, by using nCas9 to direct PolI3m to a particular genomic loci, EvolvR allows for...

... Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase Mammalian...protein of interest: you just don’t know it yet! The poly-histidine tag often used for protein purification...Green Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized...Lumio Red Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized...Lumio Red Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized...

...Mammalian TAA Simon Elsaesser 157925 pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine...azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG...

...Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional tet-responsive promoter...

...J, Plank C, Aneja MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid...

...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. This post...

...ends were modified slightly with either a 1-7 base poly(U)tail in tRNA-gRNA constructs or a 1-4 base tail...

...secondary antibody depends on whether you are using poly- or monoclonal primary antibodies. Some secondary...

...Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout Mouse Bradley...

...used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small...

...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. Return to Top...

...reading frames (ORFs) which often terminate with a poly(A). ORF2 encodes endonuclease and reverse transcriptase...

...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. Return to Top...

...be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is available ...

... OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc apoeb (apolipoprotein Eb) OZ529 and OZ530 OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg...

...civilization in general (1). Science and politics? Science is an apolitical process for seeking knowledge. The...inclusion of science to inform policy (17). The facts surrounding a matter or policy should have more weight ...science rallies scientists to political action, and inspires politicians and citizens to include science... again. No part of this process is inherently political so why is there a march in Washington, D.C., the... the seat of the United States government? Politics and science are intrinsically linked: governments ...when we drop them. Scientific realities limit what policy can do and, in this way, science has always been...been related to government and, consequently, politics. More concretely, scientists gather information ...

...Sharing Policy Discussion PLOS’ New Data Policy: Public Access to Data PLOS’ New Data Policy: Part Two...advice about the scope of the policy. End of story? Not quite. The PLOS policy on data access is a formal...clarified what the policy intent was and provided an FAQ list to further explain the policy. The fact that...year when it announced its data access and sharing policy. Since early March, the open access publisher has...the research reported in PLOS publications. The policy was not an overnight revelation, rather it was ...reasonable. PLOS has since clarified their data sharing policy and recently announced that of the 16,000 manuscripts...agencies (e.g. Wellcome Trust, NIH Data Sharing Policies, etc.) and is founded on the principle that scientific...