Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 413 results for: SaCas9

Clear keywords
Showing: 161 - 180 of 413 results

  2. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

    Enlarge

  10. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (AAV8)

    Viral Prep
    #61592-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (#61592). In addition to the viral particles, you will also receive purified pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA plasmid DNA. CMV-driven expression of SaCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Feng Zhang
    Article
    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1
    Available Since
    Sept. 22, 2025
    Availability
    Academic Institutions and Nonprofits only

  11. PRINCE-SaCas9-2

    Plasmid
    #250958
    Purpose
    PRINCE drug inducible gene editing system based on SaCas9 (AAV-Part II : the expression of 2x ERT2 and the sgRNA is under drug-mediated control)
    Depositor
    Yu Wang
    Article
    Zhang et al Sci Transl Med. 2026 May 27;18(851):eadx
    Insert
    H1-TetO-SaCas9 sgRNA scaffold; EF1α-NpuC; 2×ERT2; opTtetR; GFP
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    H1 promoter; EF1α promoter
    Available Since
    July 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    250958-plasmid-map-sequence-id-501850

    Enlarge

  15. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

    Enlarge

Showing: 161 - 180 of 413 results