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Showing: 161 - 180 of 4104 results

  3. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Availability
    Academic Institutions and Nonprofits only

  4. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.
    Depositor
    Irina Borodina
    Availability
    Academic Institutions and Nonprofits only

  6. TFORF3527

    Plasmid
    #145003
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ERF (ERF Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    145003-plasmid-map-sequence-id-287123

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  7. TFORF0959

    Plasmid
    #141766
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ERF (ERF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    141766-plasmid-map-sequence-id-388527

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  8. pDONR221_ETF1_3UTR

    Plasmid
    #65865
    Purpose
    Carries 3' UTR of ETF1, an RBPMS target
    Depositor
    Thomas Tuschl
    Insert
    ETF1 (ETF1 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  9. psicheck_ETF1_3UTR

    Plasmid
    #65884
    Purpose
    Luciferase assay vector, assessing regulation of ETF1 3' UTR
    Depositor
    Thomas Tuschl
    Insert
    ETF1 (ETF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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Showing: 161 - 180 of 4104 results